Titel: Erwinia amylovora phage vB_EamM_Y3 represents another lineage of hairy myoviridae
Autor/-in: Buttimer, Colin
Born, Yannick
Lucid, Alan
Loessner, Martin J.
Fieseler, Lars
Coffey, Aidan
et. al: No
Erschienen in: Research in Microbiology
Band(Heft): 169
Heft: 9
Seiten: 505
Seiten bis: 514
Verlag / Hrsg. Institution: Elsevier
Erscheinungsdatum: 2018
Lizenz (gemäss Verlagsvertrag): Lizenz gemäss Verlagsvertrag
Art der Begutachtung: Peer review (Publikation)
Sprache: Englisch
Schlagwörter: Erwinia amylovora; Genome; Hairy phage; Jumbo bacteriophage; Myoviridae; SAR endolysin; DNA replication; DNA, Viral; Endopeptidases; Erwinia amylovora; Myoviridae; Open reading frame; Phylogeny; DNA sequence analysis; Viral proteins; Virion; Viral genome
Fachgebiet (DDC): 570: Biologie
Zusammenfassung: To date, a small number of jumbo myoviruses have been reported to possess atypical whisker-like structures along the surface of their contractile tails. Erwinia amylovora phage vB_EamM_Y3 is another example. It possesses a genome of 261,365 kbp with 333 predicted ORFs. Using a combination of BLASTP, Interproscan and HHpred, about 21% of its putative proteins could be assigned functions involved in nucleotide metabolism, DNA replication, virion structure and cell wall degradation. The phage was found to have a signal-arrest-release (SAR) endolysin (Y3_301) possessing a soluble lytic transglycosylase domain. Like other SAR endolysins, inducible expression of Y3_301 caused Escherichia coli lysis, which is dependent on the presence of an N-terminal signal sequence. Phylogenetic analysis showed that its closest relatives are other jumbo phages including Pseudomonas aeruginosa phage PaBG and P. putida phage Lu11, sharing 105 and 87 homologous proteins respectively. Like these phages, Y3 also shares a distant relationship to Ralstonia solanacearum phage ΦRSL1 (sharing 55 homologous proteins). As these phages are unrelated to the Rak2-like group of hairy phages, Y3 along with Lu11 represent a second lineage of hairy myoviruses.
Departement: Life Sciences und Facility Management
Organisationseinheit: Institut für Lebensmittel- und Getränkeinnovation (ILGI)
Publikationstyp: Beitrag in wissenschaftlicher Zeitschrift
DOI: 10.1016/j.resmic.2018.04.006
ISSN: 0923-2508
URI: https://digitalcollection.zhaw.ch/handle/11475/18411
Publiziert im Rahmen des ZHAW-Projekts: Mechanisms of phage resistance in Erwinia amylovora
Enthalten in den Sammlungen:Publikationen Life Sciences und Facility Management

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