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Titel: The histidine-reversible antibiotic herbicolin O produced by Pantoea vagans C9-1 is pantocin A
Autoren: Ishimaru, Carol
Lansdell, Teresa A.
Clardy, Jon
Duffy, Brion
Smits, Theo H.M.
Erschienen in: Journal of Plant Pathology
Band(Heft): 99
Heft: Special issue
Seiten: 91
Seiten bis: 97
Verlag / Hrsg. Institution: Edizioni E T S
Erscheinungsdatum: Nov-2017
Sprache: Englisch / English
Schlagwörter: Fire blight; Biocontrol; Herbicolin O; Peptide antibiotic
Fachgebiet (DDC): 570: Biologie
630: Landwirtschaft
Zusammenfassung: Pantoea vagans C9-1 is one of the most effective and reliable biocontrol agents against fire blight, and has been commercialized as Blight Ban C9-1. Production of multiple antibiotics contributes to its antagonism of Erwinia amylovora. Here we describe the genetics, chemical isolation and structure of herbicolin O, the histidine-reversible antibiotic produced by P. vagans C9-1. Mutational analyses indicated that biosynthesis of herbicolin O depends on paaAB and a sequence encoding the peptide precursor of pantocin A. The paaABC gene cluster encoding biosynthesis and autoresistance was located within a 28-kb chromosomal genomic island of the complete genome sequence of P. vagans C9-1. The cluster was cloned and expressed in E. coli and purified antibiotic was isolated using improved methods for small peptides. The 1H NMR spectra of the C9-1 antibiotic closely resembled those of pantocin A produced by P. agglomerans Eh318. Detailed analysis of the proton spin systems showed that the chemical shift values and coupling constants of the protons in C9-1 herbicolin O correspond exactly to those of pantocin A. Based on these genetic and chemical analyses, herbicolin O and pantocin A are confirmed to be the same antibiotic.
Departement: Life Sciences und Facility Management
Organisationseinheit: Institut für Umwelt und Natürliche Ressourcen (IUNR)
Publikationstyp: Beitrag in wissenschaftlicher Zeitschrift / Article in scientific Journal
DOI: 10.4454/jpp.v99i0.3910
10.21256/zhaw-1520
ISSN: 2239-7264
URI: https://digitalcollection.zhaw.ch/handle/11475/1741
Enthalten in den Sammlungen:Publikationen Life Sciences und Facility Management

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