Phylogeography and population structure of the biologically invasive phytopathogen Erwinia amylovora inferred using minisatellites

Bühlmann, Andreas; Dreo, Tanja; Rezzonico, Fabio; Pothier, Joël F.; Smits, Theo H. M.; Ravnikar, Maja; Frey, Jürg E.; Duffy, Brion

doi:10.1111/1462-2920.12289

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Phylogeography and population structure of the biologically invasive phytopathogen Erwinia amylovora inferred using minisatellites
Authors:	Bühlmann, Andreas Dreo, Tanja Rezzonico, Fabio Pothier, Joël F. Smits, Theo H. M. Ravnikar, Maja Frey, Jürg E. Duffy, Brion
DOI:	10.1111/1462-2920.12289
Published in:	Environmental Microbiology
Volume(Issue):	16
Issue:	7
Page(s):	2112
Pages to:	2125
Issue Date:	Jul-2014
Publisher / Ed. Institution:	Wiley
ISSN:	1462-2912 1462-2920
Language:	English
Subject (DDC):	570: Biology
Abstract:	Erwinia amylovora causes a major disease of pome fruit trees worldwide, and is regulated as a quarantine organism in many countries. While some diversity of isolates has been observed, molecular epidemiology of this bacterium is hindered by a lack of simple molecular typing techniques with sufficiently high resolution. We report a molecular typing system of E. amylovora based on variable number of tandem repeats (VNTR) analysis. Repeats in the E. amylovora genome were identified with comparative genomic tools, and VNTR markers were developed and validated. A Multiple-Locus VNTR Analysis (MLVA) was applied to E. amylovora isolates from bacterial collections representing global and regional distribution of the pathogen. Based on six repeats, MLVA allowed the distinction of 227 haplotypes among a collection of 833 isolates of worldwide origin. Three geographically separated groups were recognized among global isolates using Bayesian clustering methods. Analysis of regional outbreaks confirmed presence of diverse haplotypes but also high representation of certain haplotypes during outbreaks. MLVA analysis is a practical method for epidemiological studies of E. amylovora, identifying previously unresolved population structure within outbreaks. Knowledge of such structure can increase our understanding on how plant diseases emerge and spread over a given geographical region.
URI:	https://digitalcollection.zhaw.ch/handle/11475/1711
Fulltext version:	Published version
License (according to publishing contract):	Licence according to publishing contract
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Natural Resource Sciences (IUNR)
Appears in collections:	Publikationen Life Sciences und Facility Management

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Bühlmann, A., Dreo, T., Rezzonico, F., Pothier, J. F., Smits, T. H. M., Ravnikar, M., Frey, J. E., & Duffy, B. (2014). Phylogeography and population structure of the biologically invasive phytopathogen Erwinia amylovora inferred using minisatellites. Environmental Microbiology, 16(7), 2112–2125. https://doi.org/10.1111/1462-2920.12289

Bühlmann, A. et al. (2014) ‘Phylogeography and population structure of the biologically invasive phytopathogen Erwinia amylovora inferred using minisatellites’, Environmental Microbiology, 16(7), pp. 2112–2125. Available at: https://doi.org/10.1111/1462-2920.12289.

A. Bühlmann et al., “Phylogeography and population structure of the biologically invasive phytopathogen Erwinia amylovora inferred using minisatellites,” Environmental Microbiology, vol. 16, no. 7, pp. 2112–2125, Jul. 2014, doi: 10.1111/1462-2920.12289.

BÜHLMANN, Andreas, Tanja DREO, Fabio REZZONICO, Joël F. POTHIER, Theo H. M. SMITS, Maja RAVNIKAR, Jürg E. FREY und Brion DUFFY, 2014. Phylogeography and population structure of the biologically invasive phytopathogen Erwinia amylovora inferred using minisatellites. Environmental Microbiology. Juli 2014. Bd. 16, Nr. 7, S. 2112–2125. DOI 10.1111/1462-2920.12289

Bühlmann, Andreas, Tanja Dreo, Fabio Rezzonico, Joël F. Pothier, Theo H. M. Smits, Maja Ravnikar, Jürg E. Frey, and Brion Duffy. 2014. “Phylogeography and Population Structure of the Biologically Invasive Phytopathogen Erwinia Amylovora Inferred Using Minisatellites.” Environmental Microbiology 16 (7): 2112–25. https://doi.org/10.1111/1462-2920.12289.

Bühlmann, Andreas, et al. “Phylogeography and Population Structure of the Biologically Invasive Phytopathogen Erwinia Amylovora Inferred Using Minisatellites.” Environmental Microbiology, vol. 16, no. 7, July 2014, pp. 2112–25, https://doi.org/10.1111/1462-2920.12289.