Title: Characterization and genetic diversity of causal agent of stone fruit bacterial canker Pseudomonas cerasi : a new pathogen of cherry
Authors : Kałużna, Monika
Willems, Anne
Pothier, Joël F.
Ruinelli, Michela
Sobiczewski, Piotr
Puławska, Joanna
Proceedings: II International Workshop on Bacterial Diseases of Stone Fruits and Nuts
Volume(Issue) : 1149
Pages : 9
Pages to: 14
Conference details: II International Workshop on Bacterial Diseases of Stone Fruits and Nuts, Izmir, Turkey, 21 April, 2015
Publisher / Ed. Institution : International Society for Horticultural Science
Issue Date: 2016
License (according to publishing contract) : Licence according to publishing contract
Type of review: Peer review (Publication)
Language : English
Subjects : Pseudomonas sp.; Bacterial canker; Diagnosis; Diversity; DNA; MLSA; MALDI TOF MS; DNA hybridization
Subject (DDC) : 570: Biology
Abstract: Based on phenotypic tests, 49 out of 168 isolates of Pseudomonas syringae obtained from various organs of diseased tissue originating from various regions in Poland, were identified as P. syringae pv. morsprunorum race 1 (Psm1), 10 as race 2 of this pathovar (Psm2), 53 as pathovar syringae (Pss) and 56 as an atypical taxon. The pathogenicity test on immature sweet cherry fruits divided the tested strains into two groups: one with isolates causing black brown necrosis and the second containing isolates inducing water soaked superficial lesions. Phenotypic and genetic studies on toxins production by bacteria showed that all Pss produced syringomycin. However, only some Psm1 isolates have the gene for coronatine production. All strains belonging to Psm2 possessed genes encoding yersiniabactin. Results of genetic analyses (rep-PCRs, PCR MP) confirmed the homogeneity of isolates within pathovar morsprunorum and the atypical taxon and revealed diversity within pathovar syringae. A detailed polyphasic approach including phenotypic and genetic characterization applied for eight strains from the atypical taxon showed that the strains represent a novel species of the genus Pseudomonas for which Pseudomonas cerasi sp. nov. (non Griffin, 1911) is proposed.
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Natural Resource Sciences (IUNR)
Publication type: Conference Paper
DOI : 10.17660/ActaHortic.2016.1149.2
ISBN: 9789462611405
ISSN: 0567-7572
URI: https://digitalcollection.zhaw.ch/handle/11475/1701
Published as part of the ZHAW project : COST Action FA1104 – Sustainable production of high quality cherries for the European market
Appears in Collections:Publikationen Life Sciences und Facility Management

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