Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-1481
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dc.contributor.authorSchneeberger, Pierre H.H.-
dc.contributor.authorPothier, Joël F.-
dc.contributor.authorBühlmann, Andreas-
dc.contributor.authorDuffy, Brion-
dc.contributor.authorBeuret, Christian-
dc.contributor.authorUtzinger, Jürg-
dc.contributor.authorFrey, Jürg E.-
dc.date.accessioned2017-12-04T14:25:26Z-
dc.date.available2017-12-04T14:25:26Z-
dc.date.issued2017-05-25-
dc.identifier.issn1932-6203de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/1678-
dc.description.abstractBackground: Viruses belonging to the Flaviviridae and Bunyaviridae families show considerable genetic diversity. However, this diversity is not necessarily taken into account when developing diagnostic assays, which are often based on the pairwise alignment of a limited number of sequences. Our objective was to develop and evaluate a bioinformatics workflow addressing two recurrent issues of molecular assay design: (i) the high intraspecies genetic diversity in viruses and (ii) the potential for cross-reactivity with close relatives. Methodology: The workflow developed herein was based on two consecutive BLASTn steps; the first was utilized to select highly conserved regions among the viral taxon of interest, and the second was employed to assess the degree of similarity of these highly-conserved regions to close relatives. Subsequently, the workflow was tested on a set of eight viral species, including various strains from the Flaviviridae and Bunyaviridae families. Principal findings: The genetic diversity ranges from as low as 0.45% variable sites over the complete genome of the Japanese encephalitis virus to more than 16% of variable sites on segment L of the Crimean-Congo hemorrhagic fever virus. Our proposed bioinformatics workflow allowed the selection – based on computing scores – of the best target for a diagnostic molecular assay for the eight viral species investigated. Conclusions/Significance: Our bioinformatics workflow allowed rapid selection of highly conserved and specific genomic fragments among the investigated viruses, while considering up to several hundred complete genomic sequences. The pertinence of this workflow will increase in parallel to the number of sequences made publicly available. We hypothesize that our workflow might be utilized to select diagnostic molecular markers for higher organisms with more complex genomes, provided the sequences are made available.de_CH
dc.language.isoende_CH
dc.publisherPublic Library of Sciencede_CH
dc.relation.ispartofPLOS ONEde_CH
dc.rightshttp://creativecommons.org/licenses/by-nc/4.0/de_CH
dc.subject.ddc570: Biologiede_CH
dc.titleDevelopment and evaluation of a bioinformatics approach for designing molecular assays for viral detectionde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Umwelt und Natürliche Ressourcen (IUNR)de_CH
dc.identifier.doi10.21256/zhaw-1481-
dc.identifier.doi10.1371/journal.pone.0178195de_CH
zhaw.funding.euNode_CH
zhaw.issue5de_CH
zhaw.originated.zhawYesde_CH
zhaw.parentwork.editorSchneider, Bradley S.-
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume12de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.webfeedUmweltgenomikde_CH
zhaw.webfeedHealth Research Hub (LSFM)de_CH
zhaw.funding.zhawIn-depth molecular analysis of stool and water samples collected in Kampala, Ugandade_CH
Appears in collections:Publikationen Life Sciences und Facility Management

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Schneeberger, P. H. H., Pothier, J. F., Bühlmann, A., Duffy, B., Beuret, C., Utzinger, J., & Frey, J. E. (2017). Development and evaluation of a bioinformatics approach for designing molecular assays for viral detection. Plos One, 12(5). https://doi.org/10.21256/zhaw-1481
Schneeberger, P.H.H. et al. (2017) ‘Development and evaluation of a bioinformatics approach for designing molecular assays for viral detection’, PLOS ONE. Edited by B.S. Schneider, 12(5). Available at: https://doi.org/10.21256/zhaw-1481.
P. H. H. Schneeberger et al., “Development and evaluation of a bioinformatics approach for designing molecular assays for viral detection,” PLOS ONE, vol. 12, no. 5, May 2017, doi: 10.21256/zhaw-1481.
SCHNEEBERGER, Pierre H.H., Joël F. POTHIER, Andreas BÜHLMANN, Brion DUFFY, Christian BEURET, Jürg UTZINGER und Jürg E. FREY, 2017. Development and evaluation of a bioinformatics approach for designing molecular assays for viral detection. Bradley S. SCHNEIDER (Hrsg.), PLOS ONE. 25 Mai 2017. Bd. 12, Nr. 5. DOI 10.21256/zhaw-1481
Schneeberger, Pierre H.H., Joël F. Pothier, Andreas Bühlmann, Brion Duffy, Christian Beuret, Jürg Utzinger, and Jürg E. Frey. 2017. “Development and Evaluation of a Bioinformatics Approach for Designing Molecular Assays for Viral Detection.” Edited by Bradley S. Schneider. Plos One 12 (5). https://doi.org/10.21256/zhaw-1481.
Schneeberger, Pierre H. H., et al. “Development and Evaluation of a Bioinformatics Approach for Designing Molecular Assays for Viral Detection.” Plos One, edited by Bradley S. Schneider, vol. 12, no. 5, May 2017, https://doi.org/10.21256/zhaw-1481.


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