|Title:||Pararhizobium polonicum sp. nov. isolated from tumors on stone fruit rootstocks|
|Authors :||Puławska, Joanna|
Pothier, Joël F.
|Published in :||Systematic and Applied Microbiology|
|Publisher / Ed. Institution :||Elsevier|
|Publisher / Ed. Institution:||Amsterdam|
|Language :||Englisch / English|
|Subjects :||Crown gall; DNA–DNA hybridization; Genome; MLSA; MALDI-TOF MS|
|Subject (DDC) :||570: Biologie / Biology|
|Abstract:||Five Gram-negative, rod-shaped, non-spore-forming bacteria were isolated from galls on different stonefruit rootstocks in Poland: strains F5.1T and F5.3 from Prunus avium F12/1, strains CP3.5 and CP17.2.1 from Prunus avium and strain AL5.1.8 from Prunus cerasifera. On the basis of 16S rDNA phylogeny, thestrains cluster together and belong to the genus Pararhizobium with type strain of Pararhizobium herbae (99.6–99.8%) as their closest relative. Phylogenetic analysis of the novel strains using housekeeping genes atpD, recA and rpoB revealed their distinct position separate from other known Rhizobium species and confirmed their relation to P. herbae. DNA–DNA hybridization of strains F5.1T, with the type strain of P. herbae LMG 25718T and Pararhizobium giardinii R-4385T revealed 28.3% and 27.9% of DNA–DNA relatedness, respectively. Phenotypic and physiological properties differentiate the novel isolates from otherclosely related species.On the basis of the results obtained, the five isolates are considered to represent a novel species of the genus Pararhizobium, for which the name Pararhizobium polonicum sp. nov. (type strain F5.1T= LMG28610T= CFBP 8359T) is proposed.|
|Departement:||Life Sciences und Facility Management|
|Organisational Unit:||Institut für Umwelt und Natürliche Ressourcen (IUNR)|
|Publication type:||Beitrag in wissenschaftlicher Zeitschrift / Article in scientific Journal|
|Type of review:||Peer review (Publikation)|
|License (according to publishing contract) :||Lizenz gemäss Verlagsvertrag / Licence according to publishing contract|
|Published as part of the ZHAW project :||COST Action FA1104 – Sustainable production of high quality cherries for the European market|
|Appears in Collections:||Publikationen Life Sciences und Facility Management|
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