|Title:||Pseudomonas cerasi sp. nov. (non Griffin, 1911) isolated from diseased tissue of cherry|
|Authors :||Kałużna, Monika|
Pothier, Joël F.
|Published in :||Systematic and Applied Microbiology|
|Publisher / Ed. Institution :||Elsevier|
|Publisher / Ed. Institution:||Amsterdam|
|Language :||Englisch / English|
|Subjects :||Pseudomonas sp; Cherry bacterial canker; DNA–DNA hybridization; Genome; MLSA; MALDI-TOF MS; FAME|
|Subject (DDC) :||570: Biologie|
|Abstract:||Eight isolates of Gram-negative fluorescent bacteria (58T, 122, 374, 791, 963, 966, 970a and 1021) were obtained from diseased tissue of cherry trees from different regions of Poland. The symptoms resembled those of bacterial canker. Based on an analysis of 16S rDNA sequences the isolates shared the highest over 99.9% similarity with Pseudomonas ficuserectae JCM 2400T and Pseudomonas congelans DSM 14939T. Phylogenetic analysis using housekeeping genes gyrB, rpoD and rpoB revealed that they form a separate cluster and confirmed their closest relation to P. syringae NCPPB 281T and P. congelans LMG 21466T. DNA–DNA hybridization between the cherry isolate 58T and the type strains of these two closely related species revealed relatedness values of 58.2% and 41.9%, respectively. This was further supported by Average Nucleotide Identity (ANIb) and Genome-to-Genome Distance (GGDC) between the whole genome sequences of strain LMG 28609T and closely related Pseudomonas species. The major cellular fatty acids are 16:0 and summed feature 3 (16:1 ω7c/15:0 iso 2OH). Phenotypic characteristics differentiated the novel isolates from other closely related species. The G + C content of the genomic DNA of strain 58T was 59%. The diversity was proved by PCR MP and BOX PCR, eliminating the possibility that they constitute a clonal population. Based on the evidence of this polyphasic taxonomic study the eight strains are considered to represent a novel species of the genus Pseudomonas for which the name Pseudomonas cerasi sp. nov. (non Griffin, 1911) is proposed. The type strain of this species is 58T (= LMG 28609T = CFBP 8305T).|
|Departement:||Life Sciences und Facility Management|
|Organisational Unit:||Institut für Umwelt und Natürliche Ressourcen (IUNR)|
|Publication type:||Beitrag in wissenschaftlicher Zeitschrift / Article in scientific Journal|
|Type of review:||Peer review (Publikation)|
|License (according to publishing contract) :||Lizenz gemäss Verlagsvertrag / Licence according to publishing contract|
|Published as part of the ZHAW project :||COST Action FA1104 – Sustainable production of high quality cherries for the European market|
|Appears in Collections:||Publikationen Life Sciences und Facility Management|
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