Titel: Pseudomonas cerasi sp. nov. (non Griffin, 1911) isolated from diseased tissue of cherry
Autor/-in: Kałużna, Monika
Willems, Anne
Pothier, Joël F.
Ruinelli, Michela
Sobiczewski, Piotr
Puławska, Joanna
Erschienen in: Systematic and Applied Microbiology
Band(Heft): 39
Heft: 6
Seiten: 370
Seiten bis: 377
Verlag / Hrsg. Institution: Elsevier
Verlag / Hrsg. Institution: Amsterdam
Erscheinungsdatum: Sep-2016
Lizenz (gemäss Verlagsvertrag): Lizenz gemäss Verlagsvertrag
Art der Begutachtung: Peer review (Publikation)
Sprache: Englisch
Schlagwörter: Pseudomonas sp; Cherry bacterial canker; DNA–DNA hybridization; Genome; MLSA; MALDI-TOF MS; FAME
Fachgebiet (DDC): 570: Biologie
Zusammenfassung: Eight isolates of Gram-negative fluorescent bacteria (58T, 122, 374, 791, 963, 966, 970a and 1021) were obtained from diseased tissue of cherry trees from different regions of Poland. The symptoms resembled those of bacterial canker. Based on an analysis of 16S rDNA sequences the isolates shared the highest over 99.9% similarity with Pseudomonas ficuserectae JCM 2400T and Pseudomonas congelans DSM 14939T. Phylogenetic analysis using housekeeping genes gyrB, rpoD and rpoB revealed that they form a separate cluster and confirmed their closest relation to P. syringae NCPPB 281T and P. congelans LMG 21466T. DNA–DNA hybridization between the cherry isolate 58T and the type strains of these two closely related species revealed relatedness values of 58.2% and 41.9%, respectively. This was further supported by Average Nucleotide Identity (ANIb) and Genome-to-Genome Distance (GGDC) between the whole genome sequences of strain LMG 28609T and closely related Pseudomonas species. The major cellular fatty acids are 16:0 and summed feature 3 (16:1 ω7c/15:0 iso 2OH). Phenotypic characteristics differentiated the novel isolates from other closely related species. The G + C content of the genomic DNA of strain 58T was 59%. The diversity was proved by PCR MP and BOX PCR, eliminating the possibility that they constitute a clonal population. Based on the evidence of this polyphasic taxonomic study the eight strains are considered to represent a novel species of the genus Pseudomonas for which the name Pseudomonas cerasi sp. nov. (non Griffin, 1911) is proposed. The type strain of this species is 58T (= LMG 28609T = CFBP 8305T).
Departement: Life Sciences und Facility Management
Organisationseinheit: Institut für Umwelt und Natürliche Ressourcen (IUNR)
Publikationstyp: Beitrag in wissenschaftlicher Zeitschrift
DOI: 10.1016/j.syapm.2016.05.005
ISSN: 0723-2020
URI: https://digitalcollection.zhaw.ch/handle/11475/1663
Publiziert im Rahmen des ZHAW-Projekts: COST Action FA1104 – Sustainable production of high quality cherries for the European market
Enthalten in den Sammlungen:Publikationen Life Sciences und Facility Management

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