Title: Dickeya solani sp. nov., a pectinolytic plant-pathogenic bacterium isolated from potato (Solanum tuberosum)
Authors : van der Wolf, Jan M.
Nijhuis, Els H.
Kowalewska, Malgorzata J.
Saddler, Gerry S.
Parkinson, Neil
Elphinstone, John G.
Pritchard, Leighton
Toth, Ian K.
Lojkowska, Ewa
Potrykus, Marta
Waleron, Malgorzata
de Vos, Paul
Cleenwerck, Ilse
Pirhonen, Minna
Garlant, Linda
Hélias, Valérie
Pothier, Joël F.
Pflüger, Valentin
Duffy, Brion
Tsror, Leah
Manulis, Shula
Published in : International Journal of Systematic and Evolutionary Microbiology
Volume(Issue) : 64
Pages : 768
Pages to: 774
Publisher / Ed. Institution : The Microbiology Society
Publisher / Ed. Institution: London
Issue Date: 1-Mar-2014
License (according to publishing contract) : Licence according to publishing contract
Type of review: Peer review (Publication)
Language : English
Subject (DDC) : 570: Biology
Abstract: Pectinolytic bacteria have been recently isolated from diseased potato plants exhibiting blackleg and slow wilt symptoms found in a number of European countries and Israel. These Gram-reaction-negative, motile, rods were identified as belonging to the genus Dickeya, previously the Pectobacterium chrysanthemi complex (Erwinia chrysanthemi), on the basis of production of a PCR product with the pelADE primers, 16S rRNA gene sequence analysis, fatty acid methyl esterase analysis, the production of phosphatases and the ability to produce indole and acids from α-methylglucoside. Differential physiological assays used previously to differentiate between strains of E. chrysanthemi, showed that these isolates belonged to biovar 3. Eight of the isolates, seven from potato and one from hyacinth, were analysed together with 21 reference strains representing all currently recognized taxa within the genus Dickeya. The novel isolates formed a distinct genetic clade in multilocus sequence analysis (MLSA) using concatenated sequences of the intergenic spacer (IGS), as well as dnaX, recA, dnaN, fusA, gapA, purA, rplB, rpoS and gyrA. Characterization by whole-cell MALDI-TOF mass spectrometry, pulsed field gel electrophoresis after digestion of whole-genome DNA with rare-cutting restriction enzymes, average nucleotide identity analysis and DNA–DNA hybridization studies, showed that although related to Dickeya dadantii, these isolates represent a novel species within the genus Dickeya, for which the name Dickeya solani sp. nov. (type strain IPO 2222T = LMG25993T = NCPPB4479T) is proposed.
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Natural Resource Sciences (IUNR)
Publication type: Article in scientific Journal
DOI : 10.1099/ijs.0.052944-0
ISSN: 1466-5026
URI: https://digitalcollection.zhaw.ch/handle/11475/1662
Published as part of the ZHAW project : MALDI-TOF MS for microorganism identification: from pattern recognition towards marker based approaches
Appears in Collections:Publikationen Life Sciences und Facility Management

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