Dickeya solani sp. nov., a pectinolytic plant-pathogenic bacterium isolated from potato (Solanum tuberosum)

van der Wolf, Jan M.; Nijhuis, Els H.; Kowalewska, Malgorzata J.; Saddler, Gerry S.; Parkinson, Neil; Elphinstone, John G.; Pritchard, Leighton; Toth, Ian K.; Lojkowska, Ewa; Potrykus, Marta; Waleron, Malgorzata; de Vos, Paul; Cleenwerck, Ilse; Pirhonen, Minna; Garlant, Linda; Hélias, Valérie; Pothier, Joël F.; Pflüger, Valentin; Duffy, Brion; Tsror, Leah; Manulis, Shula

doi:10.1099/ijs.0.052944-0

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Dickeya solani sp. nov., a pectinolytic plant-pathogenic bacterium isolated from potato (Solanum tuberosum)
Authors:	van der Wolf, Jan M. Nijhuis, Els H. Kowalewska, Malgorzata J. Saddler, Gerry S. Parkinson, Neil Elphinstone, John G. Pritchard, Leighton Toth, Ian K. Lojkowska, Ewa Potrykus, Marta Waleron, Malgorzata de Vos, Paul Cleenwerck, Ilse Pirhonen, Minna Garlant, Linda Hélias, Valérie Pothier, Joël F. Pflüger, Valentin Duffy, Brion Tsror, Leah Manulis, Shula
DOI:	10.1099/ijs.0.052944-0
Published in:	International Journal of Systematic and Evolutionary Microbiology
Volume(Issue):	64
Page(s):	768
Pages to:	774
Issue Date:	1-Mar-2014
Publisher / Ed. Institution:	Microbiology Society
Publisher / Ed. Institution:	London
ISSN:	1466-5026 1466-5034
Language:	English
Subject (DDC):	570: Biology
Abstract:	Pectinolytic bacteria have been recently isolated from diseased potato plants exhibiting blackleg and slow wilt symptoms found in a number of European countries and Israel. These Gram-reaction-negative, motile, rods were identified as belonging to the genus Dickeya, previously the Pectobacterium chrysanthemi complex (Erwinia chrysanthemi), on the basis of production of a PCR product with the pelADE primers, 16S rRNA gene sequence analysis, fatty acid methyl esterase analysis, the production of phosphatases and the ability to produce indole and acids from α-methylglucoside. Differential physiological assays used previously to differentiate between strains of E. chrysanthemi, showed that these isolates belonged to biovar 3. Eight of the isolates, seven from potato and one from hyacinth, were analysed together with 21 reference strains representing all currently recognized taxa within the genus Dickeya. The novel isolates formed a distinct genetic clade in multilocus sequence analysis (MLSA) using concatenated sequences of the intergenic spacer (IGS), as well as dnaX, recA, dnaN, fusA, gapA, purA, rplB, rpoS and gyrA. Characterization by whole-cell MALDI-TOF mass spectrometry, pulsed field gel electrophoresis after digestion of whole-genome DNA with rare-cutting restriction enzymes, average nucleotide identity analysis and DNA–DNA hybridization studies, showed that although related to Dickeya dadantii, these isolates represent a novel species within the genus Dickeya, for which the name Dickeya solani sp. nov. (type strain IPO 2222T = LMG25993T = NCPPB4479T) is proposed.
URI:	https://digitalcollection.zhaw.ch/handle/11475/1662
Fulltext version:	Published version
License (according to publishing contract):	Licence according to publishing contract
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Natural Resource Sciences (IUNR)
Published as part of the ZHAW project:	MALDI-TOF MS for microorganism identification: from pattern recognition towards marker based approaches
Appears in collections:	Publikationen Life Sciences und Facility Management

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van der Wolf, J. M., Nijhuis, E. H., Kowalewska, M. J., Saddler, G. S., Parkinson, N., Elphinstone, J. G., Pritchard, L., Toth, I. K., Lojkowska, E., Potrykus, M., Waleron, M., de Vos, P., Cleenwerck, I., Pirhonen, M., Garlant, L., Hélias, V., Pothier, J. F., Pflüger, V., Duffy, B., et al. (2014). Dickeya solani sp. nov., a pectinolytic plant-pathogenic bacterium isolated from potato (Solanum tuberosum). International Journal of Systematic and Evolutionary Microbiology, 64, 768–774. https://doi.org/10.1099/ijs.0.052944-0

van der Wolf, J.M. et al. (2014) ‘Dickeya solani sp. nov., a pectinolytic plant-pathogenic bacterium isolated from potato (Solanum tuberosum)’, International Journal of Systematic and Evolutionary Microbiology, 64, pp. 768–774. Available at: https://doi.org/10.1099/ijs.0.052944-0.

J. M. van der Wolf et al., “Dickeya solani sp. nov., a pectinolytic plant-pathogenic bacterium isolated from potato (Solanum tuberosum),” International Journal of Systematic and Evolutionary Microbiology, vol. 64, pp. 768–774, Mar. 2014, doi: 10.1099/ijs.0.052944-0.

VAN DER WOLF, Jan M., Els H. NIJHUIS, Malgorzata J. KOWALEWSKA, Gerry S. SADDLER, Neil PARKINSON, John G. ELPHINSTONE, Leighton PRITCHARD, Ian K. TOTH, Ewa LOJKOWSKA, Marta POTRYKUS, Malgorzata WALERON, Paul DE VOS, Ilse CLEENWERCK, Minna PIRHONEN, Linda GARLANT, Valérie HÉLIAS, Joël F. POTHIER, Valentin PFLÜGER, Brion DUFFY, Leah TSROR und Shula MANULIS, 2014. Dickeya solani sp. nov., a pectinolytic plant-pathogenic bacterium isolated from potato (Solanum tuberosum). International Journal of Systematic and Evolutionary Microbiology. 1 März 2014. Bd. 64, S. 768–774. DOI 10.1099/ijs.0.052944-0

van der Wolf, Jan M., Els H. Nijhuis, Malgorzata J. Kowalewska, Gerry S. Saddler, Neil Parkinson, John G. Elphinstone, Leighton Pritchard, et al. 2014. “Dickeya Solani Sp. Nov., A Pectinolytic Plant-Pathogenic Bacterium Isolated from Potato (Solanum Tuberosum).” International Journal of Systematic and Evolutionary Microbiology 64 (March): 768–74. https://doi.org/10.1099/ijs.0.052944-0.

van der Wolf, Jan M., et al. “Dickeya Solani Sp. Nov., A Pectinolytic Plant-Pathogenic Bacterium Isolated from Potato (Solanum Tuberosum).” International Journal of Systematic and Evolutionary Microbiology, vol. 64, Mar. 2014, pp. 768–74, https://doi.org/10.1099/ijs.0.052944-0.