Publication type: | Article in scientific journal |
Type of review: | Peer review (publication) |
Title: | Evaluation of a real-time PCR and a loop-mediated isothermal amplification for detection of Xanthomonas arboricola pv. pruni in plant tissue samples |
Authors: | Palacio-Bielsa, Ana López-Soriano, Pablo Bühlmann, Andreas van Doorn, Joop Pham, Khanh Cambra, Miguel A. Berruete, Isabel M. Pothier, Joël F. Duffy, Brion Olmos, Antonio López, María M. |
DOI: | 10.1016/j.mimet.2015.03.005 |
Published in: | Journal of Microbiological Methods |
Volume(Issue): | 112 |
Page(s): | 36 |
Pages to: | 39 |
Issue Date: | May-2015 |
Publisher / Ed. Institution: | Elsevier |
ISSN: | 0167-7012 |
Language: | English |
Subjects: | Xanthomonas arboricola pv. pruni; Diagnostic assays; Real-time PCR; LAMP |
Subject (DDC): | 570: Biology |
Abstract: | Operational capacity of real-time PCR and loop-mediated isothermal amplification (LAMP) diagnostic assays for detection of Xanthomonas arboricola pv. pruni was established in a ring-test involving four laboratories. Symptomatic and healthy almond leaf samples with two methods of sample preparation were analyzed. Kappa coefficient, sensitivity, specificity, likelihood ratios and post-test probability of detection were estimated to manage the risk associated with the use of the two methods. |
URI: | https://digitalcollection.zhaw.ch/handle/11475/1646 |
Fulltext version: | Published version |
License (according to publishing contract): | Licence according to publishing contract |
Departement: | Life Sciences and Facility Management |
Organisational Unit: | Institute of Natural Resource Sciences (IUNR) |
Published as part of the ZHAW project: | Comparative genomics of the plant pathogen Xanthomonas arboricola pv. pruni and related xanthomonads |
Appears in collections: | Publikationen Life Sciences und Facility Management |
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Palacio-Bielsa, A., López-Soriano, P., Bühlmann, A., van Doorn, J., Pham, K., Cambra, M. A., Berruete, I. M., Pothier, J. F., Duffy, B., Olmos, A., & López, M. M. (2015). Evaluation of a real-time PCR and a loop-mediated isothermal amplification for detection of Xanthomonas arboricola pv. pruni in plant tissue samples. Journal of Microbiological Methods, 112, 36–39. https://doi.org/10.1016/j.mimet.2015.03.005
Palacio-Bielsa, A. et al. (2015) ‘Evaluation of a real-time PCR and a loop-mediated isothermal amplification for detection of Xanthomonas arboricola pv. pruni in plant tissue samples’, Journal of Microbiological Methods, 112, pp. 36–39. Available at: https://doi.org/10.1016/j.mimet.2015.03.005.
A. Palacio-Bielsa et al., “Evaluation of a real-time PCR and a loop-mediated isothermal amplification for detection of Xanthomonas arboricola pv. pruni in plant tissue samples,” Journal of Microbiological Methods, vol. 112, pp. 36–39, May 2015, doi: 10.1016/j.mimet.2015.03.005.
PALACIO-BIELSA, Ana, Pablo LÓPEZ-SORIANO, Andreas BÜHLMANN, Joop VAN DOORN, Khanh PHAM, Miguel A. CAMBRA, Isabel M. BERRUETE, Joël F. POTHIER, Brion DUFFY, Antonio OLMOS und María M. LÓPEZ, 2015. Evaluation of a real-time PCR and a loop-mediated isothermal amplification for detection of Xanthomonas arboricola pv. pruni in plant tissue samples. Journal of Microbiological Methods. Mai 2015. Bd. 112, S. 36–39. DOI 10.1016/j.mimet.2015.03.005
Palacio-Bielsa, Ana, Pablo López-Soriano, Andreas Bühlmann, Joop van Doorn, Khanh Pham, Miguel A. Cambra, Isabel M. Berruete, et al. 2015. “Evaluation of a Real-Time PCR and a Loop-Mediated Isothermal Amplification for Detection of Xanthomonas Arboricola Pv. Pruni in Plant Tissue Samples.” Journal of Microbiological Methods 112 (May): 36–39. https://doi.org/10.1016/j.mimet.2015.03.005.
Palacio-Bielsa, Ana, et al. “Evaluation of a Real-Time PCR and a Loop-Mediated Isothermal Amplification for Detection of Xanthomonas Arboricola Pv. Pruni in Plant Tissue Samples.” Journal of Microbiological Methods, vol. 112, May 2015, pp. 36–39, https://doi.org/10.1016/j.mimet.2015.03.005.
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