|Title:||Evaluation of a real-time PCR and a loop-mediated isothermal amplification for detection of Xanthomonas arboricola pv. pruni in plant tissue samples|
|Authors :||Palacio-Bielsa, Ana|
van Doorn, Joop
Cambra, Miguel A.
Berruete, Isabel M.
Pothier, Joël F.
López, María M.
|Published in :||Journal of Microbiological Methods|
|Publisher / Ed. Institution :||Elsevier BV|
|License (according to publishing contract) :||Licence according to publishing contract|
|Type of review:||Peer review (Publication)|
|Subjects :||Xanthomonas arboricola pv. pruni; Diagnostic assays; Real-time PCR; LAMP|
|Subject (DDC) :||570: Biology|
|Abstract:||Operational capacity of real-time PCR and loop-mediated isothermal amplification (LAMP) diagnostic assays for detection of Xanthomonas arboricola pv. pruni was established in a ring-test involving four laboratories. Symptomatic and healthy almond leaf samples with two methods of sample preparation were analyzed. Kappa coefficient, sensitivity, specificity, likelihood ratios and post-test probability of detection were estimated to manage the risk associated with the use of the two methods.|
|Departement:||Life Sciences and Facility Management|
|Organisational Unit:||Institute of Natural Resource Sciences (IUNR)|
|Publication type:||Article in scientific Journal|
|Published as part of the ZHAW project :||Comparative genomics of the plant pathogen Xanthomonas arboricola pv. pruni and related xanthomonads|
|Appears in Collections:||Publikationen Life Sciences und Facility Management|
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