Title: Evaluation of a real-time PCR and a loop-mediated isothermal amplification for detection of Xanthomonas arboricola pv. pruni in plant tissue samples
Authors : Palacio-Bielsa, Ana
López-Soriano, Pablo
Bühlmann, Andreas
van Doorn, Joop
Pham, Khanh
Cambra, Miguel A.
Berruete, Isabel M.
Pothier, Joël F.
Duffy, Brion
Olmos, Antonio
López, María M.
Published in : Journal of Microbiological Methods
Volume(Issue) : 112
Pages : 36
Pages to: 39
Publisher / Ed. Institution : Elsevier BV
Issue Date: May-2015
License (according to publishing contract) : Licence according to publishing contract
Type of review: Peer review (Publication)
Language : English
Subjects : Xanthomonas arboricola pv. pruni; Diagnostic assays; Real-time PCR; LAMP
Subject (DDC) : 570: Biology
Abstract: Operational capacity of real-time PCR and loop-mediated isothermal amplification (LAMP) diagnostic assays for detection of Xanthomonas arboricola pv. pruni was established in a ring-test involving four laboratories. Symptomatic and healthy almond leaf samples with two methods of sample preparation were analyzed. Kappa coefficient, sensitivity, specificity, likelihood ratios and post-test probability of detection were estimated to manage the risk associated with the use of the two methods.
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Natural Resource Sciences (IUNR)
Publication type: Article in scientific Journal
DOI : 10.1016/j.mimet.2015.03.005
ISSN: 0167-7012
URI: https://digitalcollection.zhaw.ch/handle/11475/1646
Published as part of the ZHAW project : Comparative genomics of the plant pathogen Xanthomonas arboricola pv. pruni and related xanthomonads
Appears in Collections:Publikationen Life Sciences und Facility Management

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