Title: Xanthomonas arboricola pv. fragariae : what's in a name?
Authors : Vandroemme, Joachim
Cottyn, Bart
Pothier, Joël F.
Pflüger, Valentin
Duffy, Brion
Maes, Martine
Published in : Plant Pathology
Volume(Issue) : 62
Issue : 5
Pages : 1123
Pages to: 1131
Publisher / Ed. Institution : Wiley-Blackwell Publishing Ltd.
Issue Date: Oct-2013
Language : Englisch / English
Subject (DDC) : 570: Biologie
Abstract: Polyphasic analysis exposed important heterogeneity between bacterial strains catalogued as Xanthomonas arboricola pv. fragariae (Xaf) from different culture collections. Two draft whole-genome sequences revealed pathogenicity related genes of the type-three secretion system in strain LMG 19146, whereas none were found in the Xaf pathotype strain LMG 19145. Also, considerable sequence divergence was observed in the phylogenetic marker genes gyrB, rpoD, dnaK and fyuA. Further study of 16 Xaf culture-collection strains showed that co-classification is not justified. Partial 16S rRNA gene and gyrB sequencing demonstrated that 12 strains belonged to X. arboricola, but that they did not form one homogeneous group within the species. The four remaining strains were identified as Xanthomonas fragariae and Xanthomonas sp. All sequence-based identifications were confirmed by MALDI-TOF MS fingerprinting. Also, the pathogenicity genes hrcQ and avrBs2 were detected in only three of the 12 analysed X. arboricola strains. The X. arboricola and Xanthomonas sp. strains showed pectolytic activity, and upon inoculation in strawberry none of the strains reproduced the leaf blight symptoms reported for Xaf. This study demonstrates that (i) no clear criteria exist for the identification of strains as Xaf, (ii) the name Xaf is currently used for a genetically diverse assortment of strains, and (iii) the species X. arboricola holds many undetermined plant-associated bacteria besides the described pathovars.
Departement: Life Sciences und Facility Management
Organisational Unit: Institut für Umwelt und Natürliche Ressourcen (IUNR)
Publication type: Beitrag in wissenschaftlicher Zeitschrift / Article in scientific Journal
DOI : 10.1111/ppa.12028
ISSN: 0032-0862
URI: https://digitalcollection.zhaw.ch/handle/11475/1644
Appears in Collections:Publikationen Life Sciences und Facility Management

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