Publication type: Article in scientific journal
Type of review: Peer review (publication)
Title: Directed evolution of carbon–hydrogen bond activating enzymes
Authors: Frey, Raphael
Hayashi, Takahiro
Buller, Rebecca
DOI: 10.1016/j.copbio.2018.12.004
Published in: Current Opinion in Biotechnology
Volume(Issue): 60
Page(s): 29
Pages to: 38
Issue Date: 2019
Publisher / Ed. Institution: Elsevier
ISSN: 0958-1669
Language: English
Subject (DDC): 660.6: Biotechnology
Abstract: As industrial biocatalysis is maturing, access to enzymatic activities beyond chiral resolutions, asymmetric ketone reductions and reductive aminations is gradually becoming reality. Especially the utilization of carbon-hydrogen bond (C-H) activating enzymes is very attractive as they catalyze a variety of chemically extremely challenging transformations. Because of their intrinsic complexity, the use of these enzymes in manufacturing has been limited. However, recent advances in enzyme engineering and bioinformatics have led to activity improvements for native and non-native substrates, the introduction of new-to-nature chemistries and the identification of promising novel enzyme families. Looking forward, the use of automation and advanced computer algorithms will help to streamline the evolution process of C-H activating enzymes leading to more robust and active biocatalysts.
Fulltext version: Published version
License (according to publishing contract): Licence according to publishing contract
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:Publikationen Life Sciences und Facility Management

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