Publication type: Article in scientific journal
Type of review: Peer review (publication)
Title: Novel Old Yellow Enzyme subclasses
Authors: Peters, Christin
Frasson, David
Sievers, Martin
Buller, Rebecca
DOI: 10.1002/cbic.201800770
Published in: ChemBioChem
Issue Date: Feb-2019
Publisher / Ed. Institution: Wiley
ISSN: 1439-4227
Language: English
Subjects: Biocatalytic toolbox; Ene reductase; Enzyme sourcing; Novel Old Yellow Enzyme subclasses; Phylogenetic analysis
Subject (DDC): 660.6: Biotechnology
Abstract: Many drug candidate molecules contain at least one chiral centre and consequently, the development of biocatalytic strategies to complement existing metal- and organocatalytic approaches is of high interest. However, time is a critical factor in chemical process development and thus, the introduction of biocatalytic steps, even if more suitable, is often prevented by the limited availability of off-the-shelf enzyme libraries. To expand the biocatalytic toolbox with additional ene reductases, we screened 19 bacterial strains for double bond reduction activity using the model substrates cyclohexanone and carvone. Overall, we identified 47 genes coding for putative ene reductases. Remarkably, bioinformatic analysis of all genes and the biochemical characterization of four representative novel ene reductases led us to propose the existence of two new Old Yellow Enzyme subclasses, which we named OYE class III and class IV. Our results demonstrate that while on a DNA level each new OYE subclass features a distinct combination of sequence motifs previously known from the classical and the thermophilic-like group, their substrate scope more closely resembles the latter subclass.
Fulltext version: Published version
License (according to publishing contract): Licence according to publishing contract
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:Publikationen Life Sciences und Facility Management

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