Publikationstyp: Beitrag in wissenschaftlicher Zeitschrift
Art der Begutachtung: Peer review (Publikation)
Titel: Novel Old Yellow Enzyme subclasses
Autor/-in: Peters, Christin
Frasson, David
Sievers, Martin
Buller, Rebecca
DOI: 10.1002/cbic.201800770
Erschienen in: ChemBioChem
Erscheinungsdatum: Feb-2019
Verlag / Hrsg. Institution: Wiley
ISSN: 1439-4227
1439-7633
Sprache: Englisch
Schlagwörter: Biocatalytic toolbox; Ene reductase; Enzyme sourcing; Novel Old Yellow Enzyme subclasses; Phylogenetic analysis
Fachgebiet (DDC): 660.6: Biotechnologie
Zusammenfassung: Many drug candidate molecules contain at least one chiral centre and consequently, the development of biocatalytic strategies to complement existing metal- and organocatalytic approaches is of high interest. However, time is a critical factor in chemical process development and thus, the introduction of biocatalytic steps, even if more suitable, is often prevented by the limited availability of off-the-shelf enzyme libraries. To expand the biocatalytic toolbox with additional ene reductases, we screened 19 bacterial strains for double bond reduction activity using the model substrates cyclohexanone and carvone. Overall, we identified 47 genes coding for putative ene reductases. Remarkably, bioinformatic analysis of all genes and the biochemical characterization of four representative novel ene reductases led us to propose the existence of two new Old Yellow Enzyme subclasses, which we named OYE class III and class IV. Our results demonstrate that while on a DNA level each new OYE subclass features a distinct combination of sequence motifs previously known from the classical and the thermophilic-like group, their substrate scope more closely resembles the latter subclass.
URI: https://digitalcollection.zhaw.ch/handle/11475/16372
Volltext Version: Publizierte Version
Lizenz (gemäss Verlagsvertrag): Lizenz gemäss Verlagsvertrag
Departement: Life Sciences und Facility Management
Organisationseinheit: Institut für Chemie und Biotechnologie (ICBT)
Enthalten in den Sammlungen:Publikationen Life Sciences und Facility Management

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