In-host evolution of Staphylococcus epidermidis in a pacemaker-associated endocarditis resulting in increased antibiotic tolerance

Dengler Haunreiter, Vanina; Boumasmoud, Mathilde; Häffner, Nicola; Wipfli, Dennis; Leimer, Nadja; Rachmühl, Carole; Kühnert, Denise; Achermann, Yvonne; Zbinden, Reinhard; Benussi, Stefano; Vulin, Clement; Zinkernagel, Annelies S.

doi:10.1038/s41467-019-09053-9

Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-5506

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	In-host evolution of Staphylococcus epidermidis in a pacemaker-associated endocarditis resulting in increased antibiotic tolerance
Authors:	Dengler Haunreiter, Vanina Boumasmoud, Mathilde Häffner, Nicola Wipfli, Dennis Leimer, Nadja Rachmühl, Carole Kühnert, Denise Achermann, Yvonne Zbinden, Reinhard Benussi, Stefano Vulin, Clement Zinkernagel, Annelies S.
DOI:	10.1038/s41467-019-09053-9 10.21256/zhaw-5506
Published in:	Nature Communications
Volume(Issue):	10
Issue:	1149
Issue Date:	2019
Publisher / Ed. Institution:	Nature Publishing Group
ISSN:	2041-1723
Language:	English
Subject (DDC):	570: Biology 615: Pharmacology and therapeutics
Abstract:	Treatment failure in biofilm-associated bacterial infections is an important healthcare issue. In vitro studies and mouse models suggest that bacteria enter a slow-growing/non-growing state that results in transient tolerance to antibiotics in the absence of a specific resistance mechanism. However, little clinical confirmation of antibiotic tolerant bacteria in patients exists. In this study we investigate a Staphylococcus epidermidis pacemaker-associated endocarditis, in a patient who developed a break-through bacteremia despite taking antibiotics to which the S. epidermidis isolate is fully susceptible in vitro. Characterization of the clinical S. epidermidis isolates reveals in-host evolution over the 16-week infection period, resulting in increased antibiotic tolerance of the entire population due to a prolonged lag time until growth resumption and a reduced growth rate. Furthermore, we observe adaptation towards an increased biofilm formation capacity and genetic diversification of the S. epidermidis isolates within the patient.
URI:	https://digitalcollection.zhaw.ch/handle/11475/16117
Fulltext version:	Published version
License (according to publishing contract):	CC BY 4.0: Attribution 4.0 International
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:	Publikationen Life Sciences und Facility Management

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Dengler Haunreiter, V., Boumasmoud, M., Häffner, N., Wipfli, D., Leimer, N., Rachmühl, C., Kühnert, D., Achermann, Y., Zbinden, R., Benussi, S., Vulin, C., & Zinkernagel, A. S. (2019). In-host evolution of Staphylococcus epidermidis in a pacemaker-associated endocarditis resulting in increased antibiotic tolerance. Nature Communications, 10(1149). https://doi.org/10.1038/s41467-019-09053-9

Dengler Haunreiter, V. et al. (2019) ‘In-host evolution of Staphylococcus epidermidis in a pacemaker-associated endocarditis resulting in increased antibiotic tolerance’, Nature Communications, 10(1149). Available at: https://doi.org/10.1038/s41467-019-09053-9.

V. Dengler Haunreiter et al., “In-host evolution of Staphylococcus epidermidis in a pacemaker-associated endocarditis resulting in increased antibiotic tolerance,” Nature Communications, vol. 10, no. 1149, 2019, doi: 10.1038/s41467-019-09053-9.

DENGLER HAUNREITER, Vanina, Mathilde BOUMASMOUD, Nicola HÄFFNER, Dennis WIPFLI, Nadja LEIMER, Carole RACHMÜHL, Denise KÜHNERT, Yvonne ACHERMANN, Reinhard ZBINDEN, Stefano BENUSSI, Clement VULIN und Annelies S. ZINKERNAGEL, 2019. In-host evolution of Staphylococcus epidermidis in a pacemaker-associated endocarditis resulting in increased antibiotic tolerance. Nature Communications. 2019. Bd. 10, Nr. 1149. DOI 10.1038/s41467-019-09053-9

Dengler Haunreiter, Vanina, Mathilde Boumasmoud, Nicola Häffner, Dennis Wipfli, Nadja Leimer, Carole Rachmühl, Denise Kühnert, et al. 2019. “In-Host Evolution of Staphylococcus Epidermidis in a Pacemaker-Associated Endocarditis Resulting in Increased Antibiotic Tolerance.” Nature Communications 10 (1149). https://doi.org/10.1038/s41467-019-09053-9.

Dengler Haunreiter, Vanina, et al. “In-Host Evolution of Staphylococcus Epidermidis in a Pacemaker-Associated Endocarditis Resulting in Increased Antibiotic Tolerance.” Nature Communications, vol. 10, no. 1149, 2019, https://doi.org/10.1038/s41467-019-09053-9.