Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-1467
Title: Transcriptional profile of Salmonella enterica subsp. enterica serovar Weltevreden during alfalfa sprout colonization
Authors : Brankatschk, Kerstin
Kamber, Tim
Pothier, Joël F.
Duffy, Brion
Smits, Theo H.M.
Published in : Microbial Biotechnology
Volume(Issue) : 7
Issue : 6
Pages : 528
Pages to: 544
Publisher / Ed. Institution : Wiley-Blackwell Publishing Ltd.
Publisher / Ed. Institution: Hoboken, New Jersey
Issue Date: 29-Oct-2014
Language : Englisch / English
Subject (DDC) : 570: Biologie
Abstract: Sprouted seeds represent a great risk for infection by human enteric pathogens because of favourable growth conditions for pathogens during their germination. The aim of this study was to identify mechanisms of interactions of Salmonella enterica subsp. enterica Weltevreden with alfalfa sprouts. RNA-seq analysis of S. Weltevreden grown with sprouts in comparison with M9-glucose medium showed that among a total of 4158 annotated coding sequences, 177 genes (4.3%) and 345 genes (8.3%) were transcribed at higher levels with sprouts and in minimal medium respectively. Genes that were higher transcribed with sprouts are coding for proteins involved in mechanisms known to be important for attachment, motility and biofilm formation. Besides gene expression required for phenotypic adaption, genes involved in sulphate acquisition were higher transcribed, suggesting that the surface on alfalfa sprouts may be poor in sulphate. Genes encoding structural and effector proteins of Salmonella pathogenicity island 2, involved in survival within macrophages during infection of animal tissue, were higher transcribed with sprouts possibly as a response to environmental conditions. This study provides insight on additional mechanisms that may be important for pathogen interactions with sprouts.
Departement: Life Sciences und Facility Management
Organisational Unit: Institut für Umwelt und Natürliche Ressourcen (IUNR)
Publication type: Beitrag in wissenschaftlicher Zeitschrift / Article in scientific Journal
DOI : 10.1111/1751-7915.12104
10.21256/zhaw-1467
ISSN: 1751-7915
URI: https://digitalcollection.zhaw.ch/handle/11475/1587
Appears in Collections:Publikationen Life Sciences und Facility Management

Files in This Item:
File Description SizeFormat 
2014_Brankatschk_Transcriptional profile_MicrobBiotechnol.pdf538.89 kBAdobe PDFThumbnail
View/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.