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Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-1461
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dc.contributor.authorKamber, Tim-
dc.contributor.authorPothier, Joël-
dc.contributor.authorPelludat, Cosima-
dc.contributor.authorRezzonico, Fabio-
dc.contributor.authorDuffy, Brion-
dc.contributor.authorSmits, Theo-
dc.date.accessioned2017-11-23T12:56:31Z-
dc.date.available2017-11-23T12:56:31Z-
dc.date.issued2017-08-17-
dc.identifier.issn1471-2164de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/1557-
dc.description.abstractBackground: Type VI secretion systems (T6SS) are widespread among Gram-negative bacteria and have a potential role as essential virulence factors or to maintain symbiotic interactions. Three T6SS gene clusters were identified in the genome of E. amylovora CFBP 1430, of which T6SS-1 and T6SS-3 represent complete T6SS machineries, while T6SS-2 is reduced in its gene content. Results: To assess the contribution of T6SSs to virulence and potential transcriptomic changes of E. amylovora CFBP 1430, single and double mutants in two structural genes were generated for T6SS-1 and T6SS-3. Plant assays showed that mutants in T6SS-3 were slightly more virulent in apple shoots while inducing less disease symptoms on apple flowers, indicating that T6SSs have only a minor effect on virulence of E. amylovora CFBP 1430. The mutations led under in vitro conditions to the differential expression of type III secretion systems, iron acquisition, chemotaxis, flagellar, and fimbrial genes. Comparison of the in planta and in vitro transcriptome data sets revealed a common differential expression of three processes and a set of chemotaxis and motility genes. Additional experiments proved that T6SS mutants are impaired in their motility. Conclusion: These results suggest that the deletion of T6SSs alters metabolic and motility processes. Nevertheless, the difference in lesion development in apple shoots and flower necrosis of T6SS mutants was indicative that T6SSs influences the disease progression and the establishment of the pathogen on host plants.de_CH
dc.language.isoende_CH
dc.publisherBioMed Centralde_CH
dc.relation.ispartofBMC Genomicsde_CH
dc.rightshttp://creativecommons.org/licenses/by/4.0/de_CH
dc.subjectFire blightde_CH
dc.subjectRNA-sequencingde_CH
dc.subjectMotilityde_CH
dc.subjectFlagellade_CH
dc.subject.ddc570: Biologiede_CH
dc.titleRole of the type VI secretion systems during disease interactions of Erwinia amylovora with its plant hostde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Umwelt und Natürliche Ressourcen (IUNR)de_CH
zhaw.publisher.placeLondonde_CH
dc.identifier.doi10.21256/zhaw-1461-
dc.identifier.doi10.1186/s12864-017-4010-1de_CH
zhaw.funding.euNode_CH
zhaw.issue628de_CH
zhaw.originated.zhawYesde_CH
zhaw.pages.end12de_CH
zhaw.pages.start1de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume16de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.webfeedUmweltgenomikde_CH
zhaw.funding.zhawGenomik und Transkriptomik von Erwinia und Pantoeade_CH
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Kamber, T., Pothier, J., Pelludat, C., Rezzonico, F., Duffy, B., & Smits, T. (2017). Role of the type VI secretion systems during disease interactions of Erwinia amylovora with its plant host. BMC Genomics, 16(628), 1–12. https://doi.org/10.21256/zhaw-1461
Kamber, T. et al. (2017) ‘Role of the type VI secretion systems during disease interactions of Erwinia amylovora with its plant host’, BMC Genomics, 16(628), pp. 1–12. Available at: https://doi.org/10.21256/zhaw-1461.
T. Kamber, J. Pothier, C. Pelludat, F. Rezzonico, B. Duffy, and T. Smits, “Role of the type VI secretion systems during disease interactions of Erwinia amylovora with its plant host,” BMC Genomics, vol. 16, no. 628, pp. 1–12, Aug. 2017, doi: 10.21256/zhaw-1461.
KAMBER, Tim, Joël POTHIER, Cosima PELLUDAT, Fabio REZZONICO, Brion DUFFY und Theo SMITS, 2017. Role of the type VI secretion systems during disease interactions of Erwinia amylovora with its plant host. BMC Genomics. 17 August 2017. Bd. 16, Nr. 628, S. 1–12. DOI 10.21256/zhaw-1461
Kamber, Tim, Joël Pothier, Cosima Pelludat, Fabio Rezzonico, Brion Duffy, and Theo Smits. 2017. “Role of the Type VI Secretion Systems during Disease Interactions of Erwinia Amylovora with Its Plant Host.” BMC Genomics 16 (628): 1–12. https://doi.org/10.21256/zhaw-1461.
Kamber, Tim, et al. “Role of the Type VI Secretion Systems during Disease Interactions of Erwinia Amylovora with Its Plant Host.” BMC Genomics, vol. 16, no. 628, Aug. 2017, pp. 1–12, https://doi.org/10.21256/zhaw-1461.


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