| Publikationstyp: | Beitrag in wissenschaftlicher Zeitschrift |
| Art der Begutachtung: | Peer review (Publikation) |
| Titel: | Linear enzyme cascade for the production of (–)-iso-isopulegol |
| Autor/-in: | Peters, Christin Buller, Rebecca |
| DOI: | 10.1515/znc-2018-0146 |
| Erschienen in: | Zeitschrift für Naturforschung C |
| Band(Heft): | 74 |
| Heft: | 3-4 |
| Erscheinungsdatum: | 2019 |
| Verlag / Hrsg. Institution: | De Gruyter |
| ISSN: | 1865-7125 0939-5075 |
| Sprache: | Englisch |
| Schlagwörter: | (–)-iso-isopulegol; Biocatalysis; Ene reductases; Enzyme cascade; Squalene hopene cyclases |
| Fachgebiet (DDC): | 660.6: Biotechnologie |
| Zusammenfassung: | Biocatalysis has developed enormously in the last decade and now offers solutions for the sustainable production of chiral and highly functionalised asset molecules. Products generated by enzymatic transformations are already being used in the food, feed, chemical, pharmaceutical and cosmetic industry, and the accessible compound panoply is expected to expand even further. In particular, the combination of stereo-selective enzymes in linear cascade reactions is an elegant strategy toward enantiomeric pure compounds, as it reduces the number of isolation and purification steps and avoids accumulation of potentially unstable intermediates. Here, we present the set-up of an enzyme cascade to selectively convert citral to (-)-iso-isopulegol by combining an ene reductase and a squalene hopene cyclase. In the initial reaction step, the ene reductase YqjM from Bacillus subtilis selectively transforms citral to (S)-citronellal, which is subsequently cyclised exclusively to (-)-iso-isopulegol by a mutant of the squalene hopene cyclase from Alicyclobacillus acidocaldarius (AacSHC). With this approach, we can convert citral to an enantiopure precursor for isomenthol derivatives. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/15397 |
| Volltext Version: | Publizierte Version |
| Lizenz (gemäss Verlagsvertrag): | Lizenz gemäss Verlagsvertrag |
| Departement: | Life Sciences und Facility Management |
| Organisationseinheit: | Institut für Chemie und Biotechnologie (ICBT) |
| Enthalten in den Sammlungen: | Publikationen Life Sciences und Facility Management |
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Peters, C., & Buller, R. (2019). Linear enzyme cascade for the production of (–)-iso-isopulegol. Zeitschrift Für Naturforschung C, 74(3-4). https://doi.org/10.1515/znc-2018-0146
Peters, C. and Buller, R. (2019) ‘Linear enzyme cascade for the production of (–)-iso-isopulegol’, Zeitschrift für Naturforschung C, 74(3-4). Available at: https://doi.org/10.1515/znc-2018-0146.
C. Peters and R. Buller, “Linear enzyme cascade for the production of (–)-iso-isopulegol,” Zeitschrift für Naturforschung C, vol. 74, no. 3-4, 2019, doi: 10.1515/znc-2018-0146.
PETERS, Christin und Rebecca BULLER, 2019. Linear enzyme cascade for the production of (–)-iso-isopulegol. Zeitschrift für Naturforschung C. 2019. Bd. 74, Nr. 3-4. DOI 10.1515/znc-2018-0146
Peters, Christin, and Rebecca Buller. 2019. “Linear Enzyme Cascade for the Production Of (–)-Iso-Isopulegol.” Zeitschrift Für Naturforschung C 74 (3-4). https://doi.org/10.1515/znc-2018-0146.
Peters, Christin, and Rebecca Buller. “Linear Enzyme Cascade for the Production Of (–)-Iso-Isopulegol.” Zeitschrift Für Naturforschung C, vol. 74, no. 3-4, 2019, https://doi.org/10.1515/znc-2018-0146.
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