Linear enzyme cascade for the production of (–)-iso-isopulegol

Peters, Christin; Buller, Rebecca

doi:10.1515/znc-2018-0146

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Linear enzyme cascade for the production of (–)-iso-isopulegol
Authors:	Peters, Christin Buller, Rebecca
DOI:	10.1515/znc-2018-0146
Published in:	Zeitschrift für Naturforschung C
Volume(Issue):	74
Issue:	3-4
Issue Date:	2019
Publisher / Ed. Institution:	De Gruyter
ISSN:	1865-7125 0939-5075
Language:	English
Subjects:	(–)-iso-isopulegol; Biocatalysis; Ene reductases; Enzyme cascade; Squalene hopene cyclases
Subject (DDC):	660.6: Biotechnology
Abstract:	Biocatalysis has developed enormously in the last decade and now offers solutions for the sustainable production of chiral and highly functionalised asset molecules. Products generated by enzymatic transformations are already being used in the food, feed, chemical, pharmaceutical and cosmetic industry, and the accessible compound panoply is expected to expand even further. In particular, the combination of stereo-selective enzymes in linear cascade reactions is an elegant strategy toward enantiomeric pure compounds, as it reduces the number of isolation and purification steps and avoids accumulation of potentially unstable intermediates. Here, we present the set-up of an enzyme cascade to selectively convert citral to (-)-iso-isopulegol by combining an ene reductase and a squalene hopene cyclase. In the initial reaction step, the ene reductase YqjM from Bacillus subtilis selectively transforms citral to (S)-citronellal, which is subsequently cyclised exclusively to (-)-iso-isopulegol by a mutant of the squalene hopene cyclase from Alicyclobacillus acidocaldarius (AacSHC). With this approach, we can convert citral to an enantiopure precursor for isomenthol derivatives.
URI:	https://digitalcollection.zhaw.ch/handle/11475/15397
Fulltext version:	Published version
License (according to publishing contract):	Licence according to publishing contract
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:	Publikationen Life Sciences und Facility Management

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Peters, C., & Buller, R. (2019). Linear enzyme cascade for the production of (–)-iso-isopulegol. Zeitschrift Für Naturforschung C, 74(3-4). https://doi.org/10.1515/znc-2018-0146

Peters, C. and Buller, R. (2019) ‘Linear enzyme cascade for the production of (–)-iso-isopulegol’, Zeitschrift für Naturforschung C, 74(3-4). Available at: https://doi.org/10.1515/znc-2018-0146.

C. Peters and R. Buller, “Linear enzyme cascade for the production of (–)-iso-isopulegol,” Zeitschrift für Naturforschung C, vol. 74, no. 3-4, 2019, doi: 10.1515/znc-2018-0146.

PETERS, Christin und Rebecca BULLER, 2019. Linear enzyme cascade for the production of (–)-iso-isopulegol. Zeitschrift für Naturforschung C. 2019. Bd. 74, Nr. 3-4. DOI 10.1515/znc-2018-0146

Peters, Christin, and Rebecca Buller. 2019. “Linear Enzyme Cascade for the Production Of (–)-Iso-Isopulegol.” Zeitschrift Für Naturforschung C 74 (3-4). https://doi.org/10.1515/znc-2018-0146.

Peters, Christin, and Rebecca Buller. “Linear Enzyme Cascade for the Production Of (–)-Iso-Isopulegol.” Zeitschrift Für Naturforschung C, vol. 74, no. 3-4, 2019, https://doi.org/10.1515/znc-2018-0146.