Title: Linear enzyme cascade for the production of (–)-iso-isopulegol
Authors : Peters, Christin
Buller, Rebecca
Published in : Zeitschrift für Naturforschung C
Volume(Issue) : 74
Issue : 3-4
Publisher / Ed. Institution : De Gruyter
Issue Date: 2019
License (according to publishing contract) : Licence according to publishing contract
Type of review: Peer review (publication)
Language : English
Subjects : (–)-iso-isopulegol; Biocatalysis; Ene reductases; Enzyme cascade; Squalene hopene cyclases
Subject (DDC) : 660.6: Biotechnology
Abstract: Biocatalysis has developed enormously in the last decade and now offers solutions for the sustainable production of chiral and highly functionalised asset molecules. Products generated by enzymatic transformations are already being used in the food, feed, chemical, pharmaceutical and cosmetic industry, and the accessible compound panoply is expected to expand even further. In particular, the combination of stereo-selective enzymes in linear cascade reactions is an elegant strategy toward enantiomeric pure compounds, as it reduces the number of isolation and purification steps and avoids accumulation of potentially unstable intermediates. Here, we present the set-up of an enzyme cascade to selectively convert citral to (-)-iso-isopulegol by combining an ene reductase and a squalene hopene cyclase. In the initial reaction step, the ene reductase YqjM from Bacillus subtilis selectively transforms citral to (S)-citronellal, which is subsequently cyclised exclusively to (-)-iso-isopulegol by a mutant of the squalene hopene cyclase from Alicyclobacillus acidocaldarius (AacSHC). With this approach, we can convert citral to an enantiopure precursor for isomenthol derivatives.
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Chemistry and Biotechnology (ICBT)
Publication type: Article in scientific journal
DOI : 10.1515/znc-2018-0146
ISSN: 1865-7125
URI: https://digitalcollection.zhaw.ch/handle/11475/15397
Appears in Collections:Publikationen Life Sciences und Facility Management

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