Title: Respiratory syncytial virus subunit vaccine based on a recombinant fusion protein expressed transiently in mammalian cells
Authors : Nallet, Sophie
Amacker, Mario
Westerfeld, Nicole
Baldi, Lucia
König, Iwo
Hacker, David L.
Zaborosch, Christiane
Zurbriggen, Rinaldo
Wurm, Florian M.
Published in : Vaccine
Volume(Issue) : 27
Issue : 46
Pages : 6415
Pages to: 6419
Publisher / Ed. Institution : Elsevier
Issue Date: 2009
License (according to publishing contract) : Licence according to publishing contract
Type of review: Peer review (Publication)
Language : English
Subjects : Viral antibodies; Cell line; Inbred BALB C mice; Neutralization tests; Recombinant fusion proteins; Respiratory syncytial virus infections; Respiratory syncytial virus vaccines; Subunit vaccines; Viral fusion proteins; Virosomes; Transfection
Subject (DDC) : 615: Pharmacology and therapeutics
Abstract: Although respiratory syncytial virus (RSV) causes severe lower respiratory tract infection in infants and adults at risk, no RSV vaccine is currently available. In this report, efforts toward the generation of an RSV subunit vaccine using recombinant RSV fusion protein (rRSV-F) are described. The recombinant protein was produced by transient gene expression (TGE) in suspension-adapted human embryonic kidney cells (HEK-293E) in 4 L orbitally shaken bioreactors. It was then purified and formulated in immunostimulating reconstituted influenza virosomes (IRIVs). The candidate vaccine induced anti-RSV-F neutralizing antibodies in mice, and challenge studies in cotton rats are ongoing. If successful in preclinical and clinical trials, this will be the first recombinant subunit vaccine produced by large-scale TGE in mammalian cells.
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Chemistry and Biotechnology (ICBT)
Publication type: Article in scientific Journal
DOI : 10.1016/j.vaccine.2009.06.019
ISSN: 0264-410X
URI: https://digitalcollection.zhaw.ch/handle/11475/15015
Appears in Collections:Publikationen Life Sciences und Facility Management

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