| Publication type: | Article in scientific journal |
| Type of review: | Peer review (publication) |
| Title: | Orientational imaging of single molecules by annular illumination |
| Authors: | Sick, Beate Hecht, B. Novotny, L. |
| DOI: | 10.1103/PhysRevLett.85.4482 |
| Published in: | Physical Review Letters |
| Volume(Issue): | 85 |
| Issue: | 21 |
| Pages: | 4482 |
| Pages to: | 4485 |
| Issue Date: | 2000 |
| Publisher / Ed. Institution: | American Physical Society |
| ISSN: | 0031-9007 1079-7114 |
| Language: | English |
| Subjects: | Dipole pattern; Single molecule; Near field optics |
| Subject (DDC): | 530: Physics |
| Abstract: | The absorption dipole orientation of single fluorescent molecules is determined by mapping the spatial distribution of the squared electric field components in a high-numerical-aperture laser focus. Annular illumination geometry and the vicinity of a plane dielectric /air interface strongly enhance the longitudinal field component and the transverse fields perpendicular to the polarization direction. As a result, all three excitation field components in the focus are of comparable magnitude. The scheme holds promise to monitor rotational diffusion of single molecules in complex environments. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/14039 |
| Fulltext version: | Published version |
| License (according to publishing contract): | Licence according to publishing contract |
| Departement: | School of Engineering |
| Organisational Unit: | Institute of Data Analysis and Process Design (IDP) |
| Appears in collections: | Publikationen School of Engineering |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.