|Publication type:||Article in scientific journal|
|Type of review:||Peer review (publication)|
|Title:||Orientational imaging of single molecules by annular illumination|
|Published in:||Physical Review Letters|
|Publisher / Ed. Institution:||American Physical Society|
|Subjects:||Dipole pattern; Single molecule; Near field optics|
|Subject (DDC):||530: Physics|
|Abstract:||The absorption dipole orientation of single fluorescent molecules is determined by mapping the spatial distribution of the squared electric field components in a high-numerical-aperture laser focus. Annular illumination geometry and the vicinity of a plane dielectric /air interface strongly enhance the longitudinal field component and the transverse fields perpendicular to the polarization direction. As a result, all three excitation field components in the focus are of comparable magnitude. The scheme holds promise to monitor rotational diffusion of single molecules in complex environments.|
|Fulltext version:||Published version|
|License (according to publishing contract):||Licence according to publishing contract|
|Departement:||School of Engineering|
|Organisational Unit:||Institute of Data Analysis and Process Design (IDP)|
|Appears in collections:||Publikationen School of Engineering|
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Sick, B., Hecht, B., & Novotny, L. (2000). Orientational imaging of single molecules by annular illumination. Physical Review Letters, 85(21), 4482–4485. https://doi.org/10.1103/PhysRevLett.85.4482
Sick, B., Hecht, B. and Novotny, L. (2000) ‘Orientational imaging of single molecules by annular illumination’, Physical Review Letters, 85(21), pp. 4482–4485. Available at: https://doi.org/10.1103/PhysRevLett.85.4482.
B. Sick, B. Hecht, and L. Novotny, “Orientational imaging of single molecules by annular illumination,” Physical Review Letters, vol. 85, no. 21, pp. 4482–4485, 2000, doi: 10.1103/PhysRevLett.85.4482.
Sick, Beate, et al. “Orientational Imaging of Single Molecules by Annular Illumination.” Physical Review Letters, vol. 85, no. 21, 2000, pp. 4482–85, https://doi.org/10.1103/PhysRevLett.85.4482.
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