|Publication type:||Article in scientific journal|
|Type of review:||Peer review (publication)|
|Title:||Orientational imaging of single molecules by annular illumination|
|Published in:||Physical Review Letters|
|Publisher / Ed. Institution:||American Physical Society|
|Subjects:||Dipole pattern; Single molecule; Near field optics|
|Subject (DDC):||530: Physics|
|Abstract:||The absorption dipole orientation of single fluorescent molecules is determined by mapping the spatial distribution of the squared electric field components in a high-numerical-aperture laser focus. Annular illumination geometry and the vicinity of a plane dielectric /air interface strongly enhance the longitudinal field component and the transverse fields perpendicular to the polarization direction. As a result, all three excitation field components in the focus are of comparable magnitude. The scheme holds promise to monitor rotational diffusion of single molecules in complex environments.|
|Fulltext version:||Published version|
|License (according to publishing contract):||Licence according to publishing contract|
|Departement:||School of Engineering|
|Organisational Unit:||Institute of Data Analysis and Process Design (IDP)|
|Appears in collections:||Publikationen School of Engineering|
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.