Publication type: Article in scientific journal
Type of review: Peer review (publication)
Title: Orientational imaging of single molecules by annular illumination
Authors: Sick, Beate
Hecht, B.
Novotny, L.
DOI: 10.1103/PhysRevLett.85.4482
Published in: Physical Review Letters
Volume(Issue): 85
Issue: 21
Pages: 4482
Pages to: 4485
Issue Date: 2000
Publisher / Ed. Institution: American Physical Society
ISSN: 0031-9007
Language: English
Subjects: Dipole pattern; Single molecule; Near field optics
Subject (DDC): 530: Physics
Abstract: The absorption dipole orientation of single fluorescent molecules is determined by mapping the spatial distribution of the squared electric field components in a high-numerical-aperture laser focus. Annular illumination geometry and the vicinity of a plane dielectric /air interface strongly enhance the longitudinal field component and the transverse fields perpendicular to the polarization direction. As a result, all three excitation field components in the focus are of comparable magnitude. The scheme holds promise to monitor rotational diffusion of single molecules in complex environments.
Fulltext version: Published version
License (according to publishing contract): Licence according to publishing contract
Departement: School of Engineering
Organisational Unit: Institute of Data Analysis and Process Design (IDP)
Appears in collections:Publikationen School of Engineering

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