Publication type: | Article in scientific journal |
Type of review: | Peer review (publication) |
Title: | Orientational imaging of single molecules by annular illumination |
Authors: | Sick, Beate Hecht, B. Novotny, L. |
DOI: | 10.1103/PhysRevLett.85.4482 |
Published in: | Physical Review Letters |
Volume(Issue): | 85 |
Issue: | 21 |
Pages: | 4482 |
Pages to: | 4485 |
Issue Date: | 2000 |
Publisher / Ed. Institution: | American Physical Society |
ISSN: | 0031-9007 1079-7114 |
Language: | English |
Subjects: | Dipole pattern; Single molecule; Near field optics |
Subject (DDC): | 530: Physics |
Abstract: | The absorption dipole orientation of single fluorescent molecules is determined by mapping the spatial distribution of the squared electric field components in a high-numerical-aperture laser focus. Annular illumination geometry and the vicinity of a plane dielectric /air interface strongly enhance the longitudinal field component and the transverse fields perpendicular to the polarization direction. As a result, all three excitation field components in the focus are of comparable magnitude. The scheme holds promise to monitor rotational diffusion of single molecules in complex environments. |
URI: | https://digitalcollection.zhaw.ch/handle/11475/14039 |
Fulltext version: | Published version |
License (according to publishing contract): | Licence according to publishing contract |
Departement: | School of Engineering |
Organisational Unit: | Institute of Data Analysis and Process Design (IDP) |
Appears in collections: | Publikationen School of Engineering |
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