Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-4871
Title: Curcuma DMSO extracts and curcumin exhibit an anti-inflammatory and anti-catabolic effect on human intervertebral disc cells, possibly by influencing TLR2 expression and JNK activity
Authors : Klawitter, Marina
Quero, Lilian
Klasen, Juergen
Gloess, Alexia N.
Klopprogge, Babette
Hausmann, Oliver
Boos, Norbert
Wuertz, Karin
Published in : Journal of Inflammation
Volume(Issue) : 9
Issue : 29
Publisher / Ed. Institution : BioMed Central
Issue Date: 2012
License (according to publishing contract) : CC BY 2.0: Attribution 2.0 Generic
Type of review: Peer review (publication)
Language : English
Subject (DDC) : 580: Plants (Botany)
610: Medicine and health
Abstract: Background: As proinflammatory cytokines seem to play a role in discogenic back pain, substances exhibiting anti-inflammatory effects on intervertebral disc cells may be used as minimal-invasive therapeutics for intradiscal/epidural injection. The purpose of this study was to investigate the anti-inflammatory and anti-catabolic potential of curcuma, which has been used in the Indian Ayurvedic medicine to treat multiple ailments for a long time. Methods: Human disc cells were treated with IL-1β to induce an inflammatory/catabolic cascade. Different extracts of curcuma as well as curcumin (= a component selected based on results with curcuma extracts and HPLC/MS analysis) were tested for their ability to reduce mRNA expression of proinflammatory cytokines and matrix degrading enzymes after 6 hours (real-time RT-PCR), followed by analysis of typical inflammatory signaling mechanisms such as NF-κB (Western Blot, Transcription Factor Assay), MAP kinases (Western Blot) and Toll-like receptors (real-time RT-PCR). Quantitative data was statistically analyzed using a Mann Whitney U test with a significance level of p < 0.05 (two-tailed). Results: Results indicate that the curcuma DMSO extract significantly reduced levels of IL-6, MMP1, MMP3 and MMP13. The DMSO-soluble component curcumin, whose occurrence within the DMSO extract was verified by HPLC/MS, reduced levels of IL-1β, IL-6, IL-8, MMP1, MMP3 and MMP13 and both caused an up-regulation of TNF-α. Pathway analysis indicated that curcumin did not show involvement of NF-κB, but down-regulated TLR2 expression and inhibited the MAP kinase JNK while activating p38 and ERK. Conclusions: Based on its anti-inflammatory and anti-catabolic effects, intradiscal injection of curcumin may be an attractive treatment alternative. However, whether the anti-inflammatory properties in vitro lead to analgesia in vivo will need to be confirmed in an appropriate animal model.
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Chemistry and Biotechnology (ICBT)
Publication type: Article in scientific journal
DOI : 10.21256/zhaw-4871
10.1186/1476-9255-9-29
ISSN: 1476-9255
URI: https://digitalcollection.zhaw.ch/handle/11475/12905
Appears in Collections:Publikationen Life Sciences und Facility Management

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