|Title:||Frankia canadensis sp. nov., isolated from root nodules of Alnus incana subspecies rugosa|
|Authors :||Normand, Philippe|
Pothier, Joël F.
Fernandez, Maria P.
|Published in :||International Journal of Systematic and Evolutionary Microbiology|
|Publisher / Ed. Institution :||The Microbiology Society|
|License (according to publishing contract) :||Not specified|
|Type of review:||Peer review (Publication)|
|Subject (DDC) :||570: Biology|
|Abstract:||Strain ARgP5T, an actinobacterium isolated from a root nodule present on an Alnus incana subspecies rugosa shrub growing in Quebec City, Canada, was the subject of polyphasic taxonomic studies to clarify its status within the genus Frankia. 16S rRNA gene sequence similarities and ANI values between ARgP5T and type strains of species of the genus Frankia with validly published names were 98.8 and 82 % or less, respectively. The in silico DNA G+C content was 72.4 mol%. ARgP5T is characterised by the presence of meso-A2pm, galactose, glucose, mannose, rhamnose (trace), ribose and xylose as whole-organism hydrolysates; MK-9(H8) as predominant menaquinone; diphosphatidylglycerol, phosphatidylinositol and phosphatidylglycerol as polar lipids and iso-C16 : 0 and C17 : 1ω8c as major fatty acids. The proteomic results confirmed the distinct position of ARgP5T from its closest neighbours in Frankia cluster 1. ARgP5T was found to be infective on two alder (Alnus glutinosa and Alnusalnobetula subsp. crispa) and on one bayberry (Morella pensylvanica) species and to fix nitrogen in symbiosis and in pure culture. On the basis of phylogenetic (16S rRNA gene sequence), genomic, proteomic and phenotypic results, strain ARgP5T (=DSM 45898=CECT 9033) is considered to represent a novel species within the genus Frankia for which the name Frankia canadensis sp. nov., is proposed.|
|Departement:||Life Sciences und Facility Management|
|Organisational Unit:||Institute of Natural Resource Sciences (IUNR)|
|Publication type:||Article in scientific Journal|
|Published as part of the ZHAW project :||MALDI-TOF MS for microorganism identification: from pattern recognition towards marker based approaches|
|Appears in Collections:||Publikationen Life Sciences und Facility Management|
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