| Publication type: | Article in scientific journal |
| Type of review: | Peer review (publication) |
| Title: | Protection of Erwinia amylovora bacteriophage Y2 from UV-induced damage by natural compounds |
| Authors: | Born, Yannick Bosshard, Lars Duffy, Brion Loessner, Martin Fieseler, Lars |
| DOI: | 10.1080/21597081.2015.1074330 |
| Published in: | Bacteriophage |
| Volume(Issue): | 5 |
| Issue: | 4 |
| Page(s): | e1074330 |
| Issue Date: | Jul-2015 |
| Publisher / Ed. Institution: | Taylor & Francis |
| ISSN: | 2159-7081 2159-7073 |
| Language: | English |
| Subjects: | UV damage; Phage; Stability |
| Subject (DDC): | 570: Biology |
| Abstract: | Bacteriophages have regained much attention as biocontrol agents against bacterial pathogens. However, with respect to stability, phages are biomolecules and are therefore sensitive to a number of environmental influences. UV-irradiation can readily inactivate phage infectivity, which impedes their potential application in the plant phyllosphere. Therefore, phages for control of Erwinia amylovora, the causative agent of fire blight, need to be protected from UV-damage by adequate measures. We here investigated the protective effect of different light-absorbing substances on phage particles exposed to UV-light. For this, natural extracts from carrot, red pepper, and beetroot were prepared and tested as a natural sunscreen for phage. All compounds were found to significantly increase half-life of UV-irradiated phage particles, due to their high concentration of light absorbing carotenoids and betalaines. Pure astaxanthin, a carotenoid, also offered a significant UV-protection. The extracts themselves did not negatively affect phage viability or infectivity. Altogether, a range of readily available, natural, environmentally-safe and inexpensive substances are suitable as UV-protectants to prevent phage particles from UV-light damage. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/12264 |
| Fulltext version: | Published version |
| License (according to publishing contract): | Licence according to publishing contract |
| Departement: | Life Sciences and Facility Management |
| Organisational Unit: | Institute of Food and Beverage Innovation (ILGI) |
| Appears in collections: | Publikationen Life Sciences und Facility Management |
Files in This Item:
There are no files associated with this item.
Show full item record
Born, Y., Bosshard, L., Duffy, B., Loessner, M., & Fieseler, L. (2015). Protection of Erwinia amylovora bacteriophage Y2 from UV-induced damage by natural compounds. Bacteriophage, 5(4), e1074330. https://doi.org/10.1080/21597081.2015.1074330
Born, Y. et al. (2015) ‘Protection of Erwinia amylovora bacteriophage Y2 from UV-induced damage by natural compounds’, Bacteriophage, 5(4), p. e1074330. Available at: https://doi.org/10.1080/21597081.2015.1074330.
Y. Born, L. Bosshard, B. Duffy, M. Loessner, and L. Fieseler, “Protection of Erwinia amylovora bacteriophage Y2 from UV-induced damage by natural compounds,” Bacteriophage, vol. 5, no. 4, p. e1074330, Jul. 2015, doi: 10.1080/21597081.2015.1074330.
BORN, Yannick, Lars BOSSHARD, Brion DUFFY, Martin LOESSNER und Lars FIESELER, 2015. Protection of Erwinia amylovora bacteriophage Y2 from UV-induced damage by natural compounds. Bacteriophage. Juli 2015. Bd. 5, Nr. 4, S. e1074330. DOI 10.1080/21597081.2015.1074330
Born, Yannick, Lars Bosshard, Brion Duffy, Martin Loessner, and Lars Fieseler. 2015. “Protection of Erwinia Amylovora Bacteriophage Y2 from UV-Induced Damage by Natural Compounds.” Bacteriophage 5 (4): e1074330. https://doi.org/10.1080/21597081.2015.1074330.
Born, Yannick, et al. “Protection of Erwinia Amylovora Bacteriophage Y2 from UV-Induced Damage by Natural Compounds.” Bacteriophage, vol. 5, no. 4, July 2015, p. e1074330, https://doi.org/10.1080/21597081.2015.1074330.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.