Publication type: Article in scientific journal
Type of review: Peer review (publication)
Title: Protection of Erwinia amylovora bacteriophage Y2 from UV-induced damage by natural compounds
Authors: Born, Yannick
Bosshard, Lars
Duffy, Brion
Loessner, Martin
Fieseler, Lars
DOI: 10.1080/21597081.2015.1074330
Published in: Bacteriophage
Volume(Issue): 5
Issue: 4
Page(s): e1074330
Issue Date: Jul-2015
Publisher / Ed. Institution: Taylor & Francis
ISSN: 2159-7081
2159-7073
Language: English
Subjects: UV damage; Phage; Stability
Subject (DDC): 570: Biology
Abstract: Bacteriophages have regained much attention as biocontrol agents against bacterial pathogens. However, with respect to stability, phages are biomolecules and are therefore sensitive to a number of environmental influences. UV-irradiation can readily inactivate phage infectivity, which impedes their potential application in the plant phyllosphere. Therefore, phages for control of Erwinia¬†amylovora, the causative agent of fire blight, need to be protected from UV-damage by adequate measures. We here investigated the protective effect of different light-absorbing substances on phage particles exposed to UV-light. For this, natural extracts from carrot, red pepper, and beetroot were prepared and tested as a natural sunscreen for phage. All compounds were found to significantly increase half-life of UV-irradiated phage particles, due to their high concentration of light absorbing carotenoids and betalaines. Pure astaxanthin, a carotenoid, also offered a significant UV-protection. The extracts themselves did not negatively affect phage viability or infectivity. Altogether, a range of readily available, natural, environmentally-safe and inexpensive substances are suitable as UV-protectants to prevent phage particles from UV-light damage.
URI: https://digitalcollection.zhaw.ch/handle/11475/12264
Fulltext version: Published version
License (according to publishing contract): Licence according to publishing contract
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Food and Beverage Innovation (ILGI)
Appears in collections:Publikationen Life Sciences und Facility Management

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Born, Y., Bosshard, L., Duffy, B., Loessner, M., & Fieseler, L. (2015). Protection of Erwinia amylovora bacteriophage Y2 from UV-induced damage by natural compounds. Bacteriophage, 5(4), e1074330. https://doi.org/10.1080/21597081.2015.1074330
Born, Y. et al. (2015) ‘Protection of Erwinia amylovora bacteriophage Y2 from UV-induced damage by natural compounds’, Bacteriophage, 5(4), p. e1074330. Available at: https://doi.org/10.1080/21597081.2015.1074330.
Y. Born, L. Bosshard, B. Duffy, M. Loessner, and L. Fieseler, “Protection of Erwinia amylovora bacteriophage Y2 from UV-induced damage by natural compounds,” Bacteriophage, vol. 5, no. 4, p. e1074330, Jul. 2015, doi: 10.1080/21597081.2015.1074330.
BORN, Yannick, Lars BOSSHARD, Brion DUFFY, Martin LOESSNER und Lars FIESELER, 2015. Protection of Erwinia amylovora bacteriophage Y2 from UV-induced damage by natural compounds. Bacteriophage. Juli 2015. Bd. 5, Nr. 4, S. e1074330. DOI 10.1080/21597081.2015.1074330
Born, Yannick, Lars Bosshard, Brion Duffy, Martin Loessner, and Lars Fieseler. 2015. “Protection of Erwinia Amylovora Bacteriophage Y2 from UV-Induced Damage by Natural Compounds.” Bacteriophage 5 (4): e1074330. https://doi.org/10.1080/21597081.2015.1074330.
Born, Yannick, et al. “Protection of Erwinia Amylovora Bacteriophage Y2 from UV-Induced Damage by Natural Compounds.” Bacteriophage, vol. 5, no. 4, July 2015, p. e1074330, https://doi.org/10.1080/21597081.2015.1074330.


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