Title: Protection of Erwinia amylovora bacteriophage Y2 from UV-induced damage by natural compounds
Authors : Born, Yannick
Bosshard, Lars
Duffy, Brion
Loessner, Martin
Fieseler, Lars
Published in : Bacteriophage
Volume(Issue) : 5
Issue : 4
Pages : e1074330
Publisher / Ed. Institution : Taylor & Francis
Issue Date: Jul-2015
License (according to publishing contract) : Licence according to publishing contract
Type of review: Peer review (Publication)
Language : English
Subjects : UV damage; Phage; Stability
Subject (DDC) : 570: Biology
Abstract: Bacteriophages have regained much attention as biocontrol agents against bacterial pathogens. However, with respect to stability, phages are biomolecules and are therefore sensitive to a number of environmental influences. UV-irradiation can readily inactivate phage infectivity, which impedes their potential application in the plant phyllosphere. Therefore, phages for control of Erwinia¬†amylovora, the causative agent of fire blight, need to be protected from UV-damage by adequate measures. We here investigated the protective effect of different light-absorbing substances on phage particles exposed to UV-light. For this, natural extracts from carrot, red pepper, and beetroot were prepared and tested as a natural sunscreen for phage. All compounds were found to significantly increase half-life of UV-irradiated phage particles, due to their high concentration of light absorbing carotenoids and betalaines. Pure astaxanthin, a carotenoid, also offered a significant UV-protection. The extracts themselves did not negatively affect phage viability or infectivity. Altogether, a range of readily available, natural, environmentally-safe and inexpensive substances are suitable as UV-protectants to prevent phage particles from UV-light damage.
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Food and Beverage Innovation (ILGI)
Publication type: Article in scientific Journal
DOI : 10.1080/21597081.2015.1074330
ISSN: 2159-7081
URI: https://digitalcollection.zhaw.ch/handle/11475/12264
Appears in Collections:Publikationen Life Sciences und Facility Management

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