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dc.contributor.authorSchmitter, Sibylle-
dc.contributor.authorFieseler, Lars-
dc.contributor.authorKlumpp, Jochen-
dc.contributor.authorBertram, Ralph-
dc.contributor.authorLoessner, Martin J.-
dc.date.accessioned2018-10-29T13:42:00Z-
dc.date.available2018-10-29T13:42:00Z-
dc.date.issued2017-
dc.identifier.issn0950-382Xde_CH
dc.identifier.issn1365-2958de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/12256-
dc.description.abstractTo enable specific and tightly controlled gene expression both in vitro and during the intracellular lifecycle of the pathogen Listeria monocytogenes, a TetR-dependent genetic induction system was developed. Highest concentration of cytoplasmic TetR and best repression of tetO-controlled genes was obtained by tetR expression from the synthetic promoter Pt17. Anhydrotetracycline (ATc) as inducer permitted concentration-dependent, fine-tuned expression of genes under control of the tetO operator and a suitable promoter. The actin-polymerizing ActA protein represents a major virulence factor of L. monocytogenes, required for actin-based motility and cell-to-cell spread in infected host cells. To be able to observe its spatial and temporal distribution on intracellular L. monocytogenes cells, conditional mutants featuring actA placed under TetR control were used to infect PtK2 epithelial cells. Following induction at different time intervals, the subsequent recruitment of actin by L. monocytogenes could be monitored. We found that cells displayed functional ActA after approximately 15 min, while formation of polarized actin tail was complete after 90-120 min. At this point, intracellular motility of the induced mutants was indistinguishable from wild-type bacteria. Interestingly, de novo ActA synthesis in intracellular Listeria also demonstrated the temporal, asymmetric redistribution of the membrane-anchored proteins from the lateral walls toward the cell poles.de_CH
dc.language.isoende_CH
dc.publisherWileyde_CH
dc.relation.ispartofMolecular Microbiologyde_CH
dc.rightsLicence according to publishing contractde_CH
dc.subjectActinsde_CH
dc.subjectAnimalsde_CH
dc.subjectBacterial proteinsde_CH
dc.subjectCell culture techniquesde_CH
dc.subjectCell movementde_CH
dc.subjectCytoplasmde_CH
dc.subjectDipodomysde_CH
dc.subjectBacterial gene expression regulationde_CH
dc.subjectListeria monocytogenesde_CH
dc.subjectMembrane proteinsde_CH
dc.subjectRatsde_CH
dc.subjectSpatio-temporal analysisde_CH
dc.subjectTetracycline resistancede_CH
dc.subjectVirulence factorsde_CH
dc.subject.ddc570: Biologiede_CH
dc.titleTetR-dependent gene regulation in intracellular Listeria monocytogenes demonstrates the spatiotemporal surface distribution of ActAde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Lebensmittel- und Getränkeinnovation (ILGI)de_CH
dc.identifier.doi10.1111/mmi.13706de_CH
dc.identifier.pmid28508453de_CH
zhaw.funding.euNode_CH
zhaw.issue3de_CH
zhaw.originated.zhawYesde_CH
zhaw.pages.end425de_CH
zhaw.pages.start413de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume105de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.webfeedMikrobiologiede_CH
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Schmitter, S., Fieseler, L., Klumpp, J., Bertram, R., & Loessner, M. J. (2017). TetR-dependent gene regulation in intracellular Listeria monocytogenes demonstrates the spatiotemporal surface distribution of ActA. Molecular Microbiology, 105(3), 413–425. https://doi.org/10.1111/mmi.13706
Schmitter, S. et al. (2017) ‘TetR-dependent gene regulation in intracellular Listeria monocytogenes demonstrates the spatiotemporal surface distribution of ActA’, Molecular Microbiology, 105(3), pp. 413–425. Available at: https://doi.org/10.1111/mmi.13706.
S. Schmitter, L. Fieseler, J. Klumpp, R. Bertram, and M. J. Loessner, “TetR-dependent gene regulation in intracellular Listeria monocytogenes demonstrates the spatiotemporal surface distribution of ActA,” Molecular Microbiology, vol. 105, no. 3, pp. 413–425, 2017, doi: 10.1111/mmi.13706.
SCHMITTER, Sibylle, Lars FIESELER, Jochen KLUMPP, Ralph BERTRAM und Martin J. LOESSNER, 2017. TetR-dependent gene regulation in intracellular Listeria monocytogenes demonstrates the spatiotemporal surface distribution of ActA. Molecular Microbiology. 2017. Bd. 105, Nr. 3, S. 413–425. DOI 10.1111/mmi.13706
Schmitter, Sibylle, Lars Fieseler, Jochen Klumpp, Ralph Bertram, and Martin J. Loessner. 2017. “TetR-Dependent Gene Regulation in Intracellular Listeria Monocytogenes Demonstrates the Spatiotemporal Surface Distribution of ActA.” Molecular Microbiology 105 (3): 413–25. https://doi.org/10.1111/mmi.13706.
Schmitter, Sibylle, et al. “TetR-Dependent Gene Regulation in Intracellular Listeria Monocytogenes Demonstrates the Spatiotemporal Surface Distribution of ActA.” Molecular Microbiology, vol. 105, no. 3, 2017, pp. 413–25, https://doi.org/10.1111/mmi.13706.


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