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Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-4755
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dc.contributor.authorZeiger, Adam S.-
dc.contributor.authorLoe, Felicia C.-
dc.contributor.authorLi, Ran-
dc.contributor.authorRaghunath, Michael-
dc.contributor.authorVan Vliet, Krystyn J.-
dc.date.accessioned2018-10-29T09:23:38Z-
dc.date.available2018-10-29T09:23:38Z-
dc.date.issued2012-
dc.identifier.issn1932-6203de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/12212-
dc.description.abstractMicroenvironments of biological cells are dominated in vivo by macromolecular crowding and resultant excluded volume effects. This feature is absent in dilute in vitro cell culture. Here, we induced macromolecular crowding in vitro by using synthetic macromolecular globules of nm-scale radius at physiological levels of fractional volume occupancy. We quantified the impact of induced crowding on the extracellular and intracellular protein organization of human mesenchymal stem cells (MSCs) via immunocytochemistry, atomic force microscopy (AFM), and AFM-enabled nanoindentation. Macromolecular crowding in extracellular culture media directly induced supramolecular assembly and alignment of extracellular matrix proteins deposited by cells, which in turn increased alignment of the intracellular actin cytoskeleton. The resulting cell-matrix reciprocity further affected adhesion, proliferation, and migration behavior of MSCs. Macromolecular crowding can thus aid the design of more physiologically relevant in vitro studies and devices for MSCs and other cells, by increasing the fidelity between materials synthesized by cells in vivo and in vitro.de_CH
dc.language.isoende_CH
dc.publisherPublic Library of Sciencede_CH
dc.relation.ispartofPLOS ONEde_CH
dc.rightshttp://creativecommons.org/licenses/by/4.0/de_CH
dc.subjectWestern blottingde_CH
dc.subjectCell adhesionde_CH
dc.subjectCell culture techniquesde_CH
dc.subjectCell cyclede_CH
dc.subjectCell differentiationde_CH
dc.subjectCell movementde_CH
dc.subjectExtracellular matrixde_CH
dc.subjectFicollde_CH
dc.subjectImmunohistochemistryde_CH
dc.subjectMacromolecular substancesde_CH
dc.subjectMesenchymal stromal cellsde_CH
dc.subjectMicrobial interactionsde_CH
dc.subjectAtomic force microscopyde_CH
dc.subjectTissue engineeringde_CH
dc.subject.ddc570: Biologiede_CH
dc.titleMacromolecular crowding directs extracellular matrix organization and mesenchymal stem cell behaviorde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
dc.identifier.doi10.21256/zhaw-4755-
dc.identifier.doi10.1371/journal.pone.0037904de_CH
dc.identifier.pmid22649562de_CH
zhaw.funding.euNode_CH
zhaw.issue5de_CH
zhaw.originated.zhawNode_CH
zhaw.pages.starte37904de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume7de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.webfeedMetabolic Tissue Engineeringde_CH
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Zeiger, A. S., Loe, F. C., Li, R., Raghunath, M., & Van Vliet, K. J. (2012). Macromolecular crowding directs extracellular matrix organization and mesenchymal stem cell behavior. Plos One, 7(5), e37904. https://doi.org/10.21256/zhaw-4755
Zeiger, A.S. et al. (2012) ‘Macromolecular crowding directs extracellular matrix organization and mesenchymal stem cell behavior’, PLOS ONE, 7(5), p. e37904. Available at: https://doi.org/10.21256/zhaw-4755.
A. S. Zeiger, F. C. Loe, R. Li, M. Raghunath, and K. J. Van Vliet, “Macromolecular crowding directs extracellular matrix organization and mesenchymal stem cell behavior,” PLOS ONE, vol. 7, no. 5, p. e37904, 2012, doi: 10.21256/zhaw-4755.
ZEIGER, Adam S., Felicia C. LOE, Ran LI, Michael RAGHUNATH und Krystyn J. VAN VLIET, 2012. Macromolecular crowding directs extracellular matrix organization and mesenchymal stem cell behavior. PLOS ONE. 2012. Bd. 7, Nr. 5, S. e37904. DOI 10.21256/zhaw-4755
Zeiger, Adam S., Felicia C. Loe, Ran Li, Michael Raghunath, and Krystyn J. Van Vliet. 2012. “Macromolecular Crowding Directs Extracellular Matrix Organization and Mesenchymal Stem Cell Behavior.” Plos One 7 (5): e37904. https://doi.org/10.21256/zhaw-4755.
Zeiger, Adam S., et al. “Macromolecular Crowding Directs Extracellular Matrix Organization and Mesenchymal Stem Cell Behavior.” Plos One, vol. 7, no. 5, 2012, p. e37904, https://doi.org/10.21256/zhaw-4755.


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