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https://doi.org/10.21256/zhaw-4755| Publikationstyp: | Beitrag in wissenschaftlicher Zeitschrift |
| Art der Begutachtung: | Peer review (Publikation) |
| Titel: | Macromolecular crowding directs extracellular matrix organization and mesenchymal stem cell behavior |
| Autor/-in: | Zeiger, Adam S. Loe, Felicia C. Li, Ran Raghunath, Michael Van Vliet, Krystyn J. |
| DOI: | 10.21256/zhaw-4755 10.1371/journal.pone.0037904 |
| Erschienen in: | PLOS ONE |
| Band(Heft): | 7 |
| Heft: | 5 |
| Seite(n): | e37904 |
| Erscheinungsdatum: | 2012 |
| Verlag / Hrsg. Institution: | Public Library of Science |
| ISSN: | 1932-6203 |
| Sprache: | Englisch |
| Schlagwörter: | Western blotting; Cell adhesion; Cell culture techniques; Cell cycle; Cell differentiation; Cell movement; Extracellular matrix; Ficoll; Immunohistochemistry; Macromolecular substances; Mesenchymal stromal cells; Microbial interactions; Atomic force microscopy; Tissue engineering |
| Fachgebiet (DDC): | 570: Biologie |
| Zusammenfassung: | Microenvironments of biological cells are dominated in vivo by macromolecular crowding and resultant excluded volume effects. This feature is absent in dilute in vitro cell culture. Here, we induced macromolecular crowding in vitro by using synthetic macromolecular globules of nm-scale radius at physiological levels of fractional volume occupancy. We quantified the impact of induced crowding on the extracellular and intracellular protein organization of human mesenchymal stem cells (MSCs) via immunocytochemistry, atomic force microscopy (AFM), and AFM-enabled nanoindentation. Macromolecular crowding in extracellular culture media directly induced supramolecular assembly and alignment of extracellular matrix proteins deposited by cells, which in turn increased alignment of the intracellular actin cytoskeleton. The resulting cell-matrix reciprocity further affected adhesion, proliferation, and migration behavior of MSCs. Macromolecular crowding can thus aid the design of more physiologically relevant in vitro studies and devices for MSCs and other cells, by increasing the fidelity between materials synthesized by cells in vivo and in vitro. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/12212 |
| Volltext Version: | Publizierte Version |
| Lizenz (gemäss Verlagsvertrag): | CC BY 4.0: Namensnennung 4.0 International |
| Departement: | Life Sciences und Facility Management |
| Organisationseinheit: | Institut für Chemie und Biotechnologie (ICBT) |
| Enthalten in den Sammlungen: | Publikationen Life Sciences und Facility Management |
Dateien zu dieser Ressource:
| Datei | Beschreibung | Größe | Format | |
|---|---|---|---|---|
| 2012_Zeiger_et_al_Macromolecular_crowding_directs.PDF | 1.31 MB | Adobe PDF |  Öffnen/Anzeigen |
Zur Langanzeige
Zeiger, A. S., Loe, F. C., Li, R., Raghunath, M., & Van Vliet, K. J. (2012). Macromolecular crowding directs extracellular matrix organization and mesenchymal stem cell behavior. Plos One, 7(5), e37904. https://doi.org/10.21256/zhaw-4755
Zeiger, A.S. et al. (2012) ‘Macromolecular crowding directs extracellular matrix organization and mesenchymal stem cell behavior’, PLOS ONE, 7(5), p. e37904. Available at: https://doi.org/10.21256/zhaw-4755.
A. S. Zeiger, F. C. Loe, R. Li, M. Raghunath, and K. J. Van Vliet, “Macromolecular crowding directs extracellular matrix organization and mesenchymal stem cell behavior,” PLOS ONE, vol. 7, no. 5, p. e37904, 2012, doi: 10.21256/zhaw-4755.
ZEIGER, Adam S., Felicia C. LOE, Ran LI, Michael RAGHUNATH und Krystyn J. VAN VLIET, 2012. Macromolecular crowding directs extracellular matrix organization and mesenchymal stem cell behavior. PLOS ONE. 2012. Bd. 7, Nr. 5, S. e37904. DOI 10.21256/zhaw-4755
Zeiger, Adam S., Felicia C. Loe, Ran Li, Michael Raghunath, and Krystyn J. Van Vliet. 2012. “Macromolecular Crowding Directs Extracellular Matrix Organization and Mesenchymal Stem Cell Behavior.” Plos One 7 (5): e37904. https://doi.org/10.21256/zhaw-4755.
Zeiger, Adam S., et al. “Macromolecular Crowding Directs Extracellular Matrix Organization and Mesenchymal Stem Cell Behavior.” Plos One, vol. 7, no. 5, 2012, p. e37904, https://doi.org/10.21256/zhaw-4755.
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