In vitro evaluation of Ficoll‐enriched and genipin‐stabilised collagen scaffolds

Satyam, A.; Subramanian, G. S.; Raghunath, M.; Pandit, A.; Zeugolis, D. I.

doi:10.1002/term.1522

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	In vitro evaluation of Ficoll‐enriched and genipin‐stabilised collagen scaffolds
Authors:	Satyam, A. Subramanian, G. S. Raghunath, M. Pandit, A. Zeugolis, D. I.
DOI:	10.1002/term.1522
Published in:	Journal of Tissue Engineering and Regenerative Medicine
Volume(Issue):	8
Issue:	3
Page(s):	233
Pages to:	241
Issue Date:	May-2012
Publisher / Ed. Institution:	Wiley
ISSN:	1932-7005
Language:	English
Subject (DDC):	572: Biochemistry 610: Medicine and health
Abstract:	Polysaccharides are frequently incorporated into scaffolds for tissue engineering applications to improve mechanical and biological properties. We evaluated the influence of a Ficoll® scaffold on collagen films, a scaffold that is extensively used for soft and hard tissue repair. To avoid cytotoxicity issues associated with chemical reagents, the influence of genipin, a naturally occurring crosslinking agent, was assessed. Ultra‐structural level collagen films formed with and without Ficoll showed a fine fibrillar structure whereas genipin crosslinked films showed a coarse fibrillar and partially nodular structure. In contrast, glutaraldehyde crosslinked films lost their fibrillar pattern. Crosslinking significantly increased denaturation temperature (p < 0.001), stress (p < 0.0001) and force (p < 0.0001) at break. Collagen/Ficoll and collagen/Ficoll/genipin films showed the highest WI38 fibroblast attachment than any other scaffold (p < 0.003) and significantly greater WI38 fibroblast metabolic activity than other scaffolds (p < 0.001). By day 6. collagen/Ficoll/genipin films also induced higher and more aligned fibronectin matrix deposition than other scaffolds. Overall, this study indicates the suitability of collagen/Ficoll/genipin for tissue engineering applications.
URI:	https://digitalcollection.zhaw.ch/handle/11475/12211
Fulltext version:	Published version
License (according to publishing contract):	Licence according to publishing contract
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:	Publikationen Life Sciences und Facility Management

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Satyam, A., Subramanian, G. S., Raghunath, M., Pandit, A., & Zeugolis, D. I. (2012). In vitro evaluation of Ficoll‐enriched and genipin‐stabilised collagen scaffolds. Journal of Tissue Engineering and Regenerative Medicine, 8(3), 233–241. https://doi.org/10.1002/term.1522

Satyam, A. et al. (2012) ‘In vitro evaluation of Ficoll‐enriched and genipin‐stabilised collagen scaffolds’, Journal of Tissue Engineering and Regenerative Medicine, 8(3), pp. 233–241. Available at: https://doi.org/10.1002/term.1522.

A. Satyam, G. S. Subramanian, M. Raghunath, A. Pandit, and D. I. Zeugolis, “In vitro evaluation of Ficoll‐enriched and genipin‐stabilised collagen scaffolds,” Journal of Tissue Engineering and Regenerative Medicine, vol. 8, no. 3, pp. 233–241, May 2012, doi: 10.1002/term.1522.

SATYAM, A., G. S. SUBRAMANIAN, M. RAGHUNATH, A. PANDIT und D. I. ZEUGOLIS, 2012. In vitro evaluation of Ficoll‐enriched and genipin‐stabilised collagen scaffolds. Journal of Tissue Engineering and Regenerative Medicine. Mai 2012. Bd. 8, Nr. 3, S. 233–241. DOI 10.1002/term.1522

Satyam, A., G. S. Subramanian, M. Raghunath, A. Pandit, and D. I. Zeugolis. 2012. “In Vitro Evaluation of Ficoll‐Enriched and Genipin‐Stabilised Collagen Scaffolds.” Journal of Tissue Engineering and Regenerative Medicine 8 (3): 233–41. https://doi.org/10.1002/term.1522.

Satyam, A., et al. “In Vitro Evaluation of Ficoll‐Enriched and Genipin‐Stabilised Collagen Scaffolds.” Journal of Tissue Engineering and Regenerative Medicine, vol. 8, no. 3, May 2012, pp. 233–41, https://doi.org/10.1002/term.1522.