| Publikationstyp: | Beitrag in wissenschaftlicher Zeitschrift |
| Art der Begutachtung: | Peer review (Publikation) |
| Titel: | Applying macromolecular crowding to enhance extracellular matrix deposition and its remodeling in vitro for tissue engineering and cell-based therapies |
| Autor/-in: | Chen, Clarice Loe, Felicia Blocki, Anna Peng, Yanxian Raghunath, Michael |
| DOI: | 10.1016/j.addr.2011.03.003 |
| Erschienen in: | Advanced Drug Delivery Reviews |
| Band(Heft): | 63 |
| Heft: | 4-5 |
| Seite(n): | 277 |
| Seiten bis: | 290 |
| Erscheinungsdatum: | 2011 |
| Verlag / Hrsg. Institution: | Elsevier |
| ISSN: | 0169-409X 1872-8294 |
| Sprache: | Englisch |
| Fachgebiet (DDC): | 570: Biologie 610: Medizin und Gesundheit |
| Zusammenfassung: | With the advent of multicellular organisms, the exterior of the cells evolved dramatically from highly aqueous surroundings into an extracellular matrix and space crowded with macromolecules. Cell-based therapies require removal of cells from their crowded physiological context and propagating them in dilute culture medium to attain therapeutically relevant numbers whilst preserving their phenotype. However, bereft of their microenvironment, cells under perform and lose functionality. Major efforts currently aim to modify cell culture surfaces and build three dimensional scaffolds to improve this situation. We discuss here alternative strategies that enable cells to re-create their own microenvironment in vitro, using carbohydrate-based macromolecules as culture media additives that create an excluded volume effect at defined fraction volume occupancies. This biophysical approach dramatically enhances extracellular matrix deposition by differentiated cells and stem cells, and boosts progenitor cell differentiation and proliferation. We begin to understand how well cells really can perform ex vivo if given the chance. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/12210 |
| Volltext Version: | Publizierte Version |
| Lizenz (gemäss Verlagsvertrag): | Lizenz gemäss Verlagsvertrag |
| Departement: | Life Sciences und Facility Management |
| Organisationseinheit: | Institut für Chemie und Biotechnologie (ICBT) |
| Enthalten in den Sammlungen: | Publikationen Life Sciences und Facility Management |
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Chen, C., Loe, F., Blocki, A., Peng, Y., & Raghunath, M. (2011). Applying macromolecular crowding to enhance extracellular matrix deposition and its remodeling in vitro for tissue engineering and cell-based therapies. Advanced Drug Delivery Reviews, 63(4-5), 277–290. https://doi.org/10.1016/j.addr.2011.03.003
Chen, C. et al. (2011) ‘Applying macromolecular crowding to enhance extracellular matrix deposition and its remodeling in vitro for tissue engineering and cell-based therapies’, Advanced Drug Delivery Reviews, 63(4-5), pp. 277–290. Available at: https://doi.org/10.1016/j.addr.2011.03.003.
C. Chen, F. Loe, A. Blocki, Y. Peng, and M. Raghunath, “Applying macromolecular crowding to enhance extracellular matrix deposition and its remodeling in vitro for tissue engineering and cell-based therapies,” Advanced Drug Delivery Reviews, vol. 63, no. 4-5, pp. 277–290, 2011, doi: 10.1016/j.addr.2011.03.003.
CHEN, Clarice, Felicia LOE, Anna BLOCKI, Yanxian PENG und Michael RAGHUNATH, 2011. Applying macromolecular crowding to enhance extracellular matrix deposition and its remodeling in vitro for tissue engineering and cell-based therapies. Advanced Drug Delivery Reviews. 2011. Bd. 63, Nr. 4-5, S. 277–290. DOI 10.1016/j.addr.2011.03.003
Chen, Clarice, Felicia Loe, Anna Blocki, Yanxian Peng, and Michael Raghunath. 2011. “Applying Macromolecular Crowding to Enhance Extracellular Matrix Deposition and Its Remodeling in Vitro for Tissue Engineering and Cell-Based Therapies.” Advanced Drug Delivery Reviews 63 (4-5): 277–90. https://doi.org/10.1016/j.addr.2011.03.003.
Chen, Clarice, et al. “Applying Macromolecular Crowding to Enhance Extracellular Matrix Deposition and Its Remodeling in Vitro for Tissue Engineering and Cell-Based Therapies.” Advanced Drug Delivery Reviews, vol. 63, no. 4-5, 2011, pp. 277–90, https://doi.org/10.1016/j.addr.2011.03.003.
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