Title: Applying macromolecular crowding to enhance extracellular matrix deposition and its remodeling in vitro for tissue engineering and cell-based therapies
Authors : Chen, Clarice
Loe, Felicia
Blocki, Anna
Peng, Yanxian
Raghunath, Michael
Published in : Advanced drug delivery reviews
Volume(Issue) : 63
Issue : 4-5
Pages : 277
Pages to: 290
Publisher / Ed. Institution : Elsevier
Issue Date: 2011
License (according to publishing contract) : Licence according to publishing contract
Type of review: Peer review (Publication)
Language : English
Subject (DDC) : 570: Biology
610: Medicine and health
Abstract: With the advent of multicellular organisms, the exterior of the cells evolved dramatically from highly aqueous surroundings into an extracellular matrix and space crowded with macromolecules. Cell-based therapies require removal of cells from their crowded physiological context and propagating them in dilute culture medium to attain therapeutically relevant numbers whilst preserving their phenotype. However, bereft of their microenvironment, cells under perform and lose functionality. Major efforts currently aim to modify cell culture surfaces and build three dimensional scaffolds to improve this situation. We discuss here alternative strategies that enable cells to re-create their own microenvironment in vitro, using carbohydrate-based macromolecules as culture media additives that create an excluded volume effect at defined fraction volume occupancies. This biophysical approach dramatically enhances extracellular matrix deposition by differentiated cells and stem cells, and boosts progenitor cell differentiation and proliferation. We begin to understand how well cells really can perform ex vivo if given the chance.
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Chemistry and Biotechnology (ICBT)
Publication type: Article in scientific Journal
DOI : 10.1016/j.addr.2011.03.003
ISSN: 0169-409X
1872-8294
URI: https://digitalcollection.zhaw.ch/handle/11475/12210
Appears in Collections:Publikationen Life Sciences und Facility Management

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