| Publication type: | Article in scientific journal |
| Type of review: | Peer review (publication) |
| Title: | Collagen solubility testing, a quality assurance step for reproducible electro-spun nano-fibre fabrication : a technical note |
| Authors: | Zeugolis, D. I. Li, B. Lareu, R.R. Chan, C.K. Raghunath, M. |
| DOI: | 10.1163/156856208786052344 |
| Published in: | Journal of Biomaterials Science, Polymer Edition |
| Volume(Issue): | 19 |
| Issue: | 10 |
| Page(s): | 1307 |
| Pages to: | 1317 |
| Issue Date: | 2008 |
| Publisher / Ed. Institution: | Taylor & Francis |
| ISSN: | 0920-5063 1568-5624 |
| Language: | English |
| Subject (DDC): | 572: Biochemistry 620: Engineering |
| Abstract: | Collagen is the main component of the extra-cellular matrix and has been utilised for numerous clinical applications in many forms and products. However, since collagen remains a natural animal-derived biopolymer, variation between batches should be addressed and minimised to ensure reproducibility of the fabrication process. Recently, electro-spinning of collagen has been introduced as a leading technique for the production of bio-mimetic nano-scale scaffolds for tissue-engineering applications. However, no protocols are available that would allow comparisons of the quality of different collagen raw materials prior to the electro-spinning process. In order to bridge this gap we assessed the solubility of various freeze-dried collagens in 0.5 M acetic acid and analysed the solved collagen by gel electrophoresis. We show that raw material of limited solubility in acetic acid will not render high quality electro-spun nano-fibres using hexafluoropropanol. In particular, insoluble collagen directly failed to produce nano-fibres, collagen of reduced solubility produced fused nano-fibres with limited inter-nano-fibre space, whilst purified type-I collagen of high solubility produced smooth, reproducible nano-fibres. Gel electrophoresis confirmed the amount of solubility, as well as qualitative differences in terms of collagen cross-links and collagen types. We recommend this simple and fast step to save costs and to enhance control over the electro-spinning process of collagen. Furthermore, we believe that the solubility test should be introduced prior to any collagenous matrix preparation in order to ensure reproducibility and accuracy. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/12208 |
| Fulltext version: | Published version |
| License (according to publishing contract): | Licence according to publishing contract |
| Departement: | Life Sciences and Facility Management |
| Organisational Unit: | Institute of Chemistry and Biotechnology (ICBT) |
| Appears in collections: | Publikationen Life Sciences und Facility Management |
Files in This Item:
There are no files associated with this item.
Show full item record
Zeugolis, D. I., Li, B., Lareu, R. R., Chan, C. K., & Raghunath, M. (2008). Collagen solubility testing, a quality assurance step for reproducible electro-spun nano-fibre fabrication : a technical note. Journal of Biomaterials Science, Polymer Edition, 19(10), 1307–1317. https://doi.org/10.1163/156856208786052344
Zeugolis, D.I. et al. (2008) ‘Collagen solubility testing, a quality assurance step for reproducible electro-spun nano-fibre fabrication : a technical note’, Journal of Biomaterials Science, Polymer Edition, 19(10), pp. 1307–1317. Available at: https://doi.org/10.1163/156856208786052344.
D. I. Zeugolis, B. Li, R. R. Lareu, C. K. Chan, and M. Raghunath, “Collagen solubility testing, a quality assurance step for reproducible electro-spun nano-fibre fabrication : a technical note,” Journal of Biomaterials Science, Polymer Edition, vol. 19, no. 10, pp. 1307–1317, 2008, doi: 10.1163/156856208786052344.
ZEUGOLIS, D. I., B. LI, R.R. LAREU, C.K. CHAN und M. RAGHUNATH, 2008. Collagen solubility testing, a quality assurance step for reproducible electro-spun nano-fibre fabrication : a technical note. Journal of Biomaterials Science, Polymer Edition. 2008. Bd. 19, Nr. 10, S. 1307–1317. DOI 10.1163/156856208786052344
Zeugolis, D. I., B. Li, R.R. Lareu, C.K. Chan, and M. Raghunath. 2008. “Collagen Solubility Testing, a Quality Assurance Step for Reproducible Electro-Spun Nano-Fibre Fabrication : A Technical Note.” Journal of Biomaterials Science, Polymer Edition 19 (10): 1307–17. https://doi.org/10.1163/156856208786052344.
Zeugolis, D. I., et al. “Collagen Solubility Testing, a Quality Assurance Step for Reproducible Electro-Spun Nano-Fibre Fabrication : A Technical Note.” Journal of Biomaterials Science, Polymer Edition, vol. 19, no. 10, 2008, pp. 1307–17, https://doi.org/10.1163/156856208786052344.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.