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dc.contributor.authorRaghunath, Michael-
dc.contributor.authorWong, Yuan Sy-
dc.contributor.authorFarooq, Muhammad-
dc.contributor.authorGee, Ruowen-
dc.date.accessioned2018-10-26T14:44:50Z-
dc.date.available2018-10-26T14:44:50Z-
dc.date.issued2009-
dc.identifier.issn0006-291Xde_CH
dc.identifier.issn1090-2104de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/12206-
dc.description.abstractThe rapid vascularisation of biomaterials and engineered tissue after implantation is a current unmet need. To this end, we explored the pharmacological option of inducing neovascularisation using compounds that inhibit hypoxia-induced factor-1α prolyl hydroxylase. This stabilises hypoxia inducible factor-1α and therefore de-repress the transcription of various angiogenic genes. In the quest for a small vertebrate model allowing for in vivo screening we exposed transgenic zebrafish embryos exhibiting fluorescent blood vessels to hydralazine hydrochloride and 2,4-pyridine dicarboxylic acid from 6 hpf to 72 hpf by immersion. Live observation of embryos revealed that the substances induced formation of ectopic blood vessels in the subintestinal vessel basket. We confirmed the HIF-stabilising effects biochemically in human fibroblasts and with an in vitro angiogenesis fibroblast/HUVEC co-culture model. Cross-inhibition of collagen prolyl hydroxylase was confirmed by reduced collagen secretion by fibroblasts and reduced collagen content of zebrafish embryos.de_CH
dc.language.isoende_CH
dc.publisherElsevierde_CH
dc.relation.ispartofBiochemical and Biophysical Research Communicationsde_CH
dc.rightsLicence according to publishing contractde_CH
dc.subject.ddc571: Physiologie und verwandte Themende_CH
dc.subject.ddc572: Biochemiede_CH
dc.titlePharmacologically induced angiogenesis in transgenic zebrafishde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
dc.identifier.doi10.1016/j.bbrc.2008.11.127de_CH
zhaw.funding.euNode_CH
zhaw.issue4de_CH
zhaw.originated.zhawNode_CH
zhaw.pages.end771de_CH
zhaw.pages.start766de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume378de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.webfeedMetabolic Tissue Engineeringde_CH
Appears in collections:Publikationen Life Sciences und Facility Management

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Raghunath, M., Wong, Y. S., Farooq, M., & Gee, R. (2009). Pharmacologically induced angiogenesis in transgenic zebrafish. Biochemical and Biophysical Research Communications, 378(4), 766–771. https://doi.org/10.1016/j.bbrc.2008.11.127
Raghunath, M. et al. (2009) ‘Pharmacologically induced angiogenesis in transgenic zebrafish’, Biochemical and Biophysical Research Communications, 378(4), pp. 766–771. Available at: https://doi.org/10.1016/j.bbrc.2008.11.127.
M. Raghunath, Y. S. Wong, M. Farooq, and R. Gee, “Pharmacologically induced angiogenesis in transgenic zebrafish,” Biochemical and Biophysical Research Communications, vol. 378, no. 4, pp. 766–771, 2009, doi: 10.1016/j.bbrc.2008.11.127.
RAGHUNATH, Michael, Yuan Sy WONG, Muhammad FAROOQ und Ruowen GEE, 2009. Pharmacologically induced angiogenesis in transgenic zebrafish. Biochemical and Biophysical Research Communications. 2009. Bd. 378, Nr. 4, S. 766–771. DOI 10.1016/j.bbrc.2008.11.127
Raghunath, Michael, Yuan Sy Wong, Muhammad Farooq, and Ruowen Gee. 2009. “Pharmacologically Induced Angiogenesis in Transgenic Zebrafish.” Biochemical and Biophysical Research Communications 378 (4): 766–71. https://doi.org/10.1016/j.bbrc.2008.11.127.
Raghunath, Michael, et al. “Pharmacologically Induced Angiogenesis in Transgenic Zebrafish.” Biochemical and Biophysical Research Communications, vol. 378, no. 4, 2009, pp. 766–71, https://doi.org/10.1016/j.bbrc.2008.11.127.


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