| Title: | Pharmacologically induced angiogenesis in transgenic zebrafish |
| Authors : | Raghunath, Michael Wong, Yuan Sy Farooq, Muhammad Gee, Ruowen |
| Published in : | Biochemical and biophysical research communications |
| Volume(Issue) : | 378 |
| Issue : | 4 |
| Pages : | 766 |
| Pages to: | 771 |
| Publisher / Ed. Institution : | Elsevier |
| Issue Date: | 2009 |
| License (according to publishing contract) : | Licence according to publishing contract |
| Type of review: | Peer review (Publication) |
| Language : | English |
| Subject (DDC) : | 571: Physiology and related subjects 572: Biochemistry |
| Abstract: | The rapid vascularisation of biomaterials and engineered tissue after implantation is a current unmet need. To this end, we explored the pharmacological option of inducing neovascularisation using compounds that inhibit hypoxia-induced factor-1α prolyl hydroxylase. This stabilises hypoxia inducible factor-1α and therefore de-repress the transcription of various angiogenic genes. In the quest for a small vertebrate model allowing for in vivo screening we exposed transgenic zebrafish embryos exhibiting fluorescent blood vessels to hydralazine hydrochloride and 2,4-pyridine dicarboxylic acid from 6 hpf to 72 hpf by immersion. Live observation of embryos revealed that the substances induced formation of ectopic blood vessels in the subintestinal vessel basket. We confirmed the HIF-stabilising effects biochemically in human fibroblasts and with an in vitro angiogenesis fibroblast/HUVEC co-culture model. Cross-inhibition of collagen prolyl hydroxylase was confirmed by reduced collagen secretion by fibroblasts and reduced collagen content of zebrafish embryos. |
| Departement: | Life Sciences and Facility Management |
| Organisational Unit: | Institute of Chemistry and Biotechnology (ICBT) |
| Publication type: | Article in scientific Journal |
| DOI : | 10.1016/j.bbrc.2008.11.127 |
| ISSN: | 0006-291X 1090-2104 |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/12206 |
| Appears in Collections: | Publikationen Life Sciences und Facility Management |
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