Publication type: | Article in scientific journal |
Type of review: | Peer review (publication) |
Title: | Pharmacologically induced angiogenesis in transgenic zebrafish |
Authors: | Raghunath, Michael Wong, Yuan Sy Farooq, Muhammad Gee, Ruowen |
DOI: | 10.1016/j.bbrc.2008.11.127 |
Published in: | Biochemical and Biophysical Research Communications |
Volume(Issue): | 378 |
Issue: | 4 |
Pages: | 766 |
Pages to: | 771 |
Issue Date: | 2009 |
Publisher / Ed. Institution: | Elsevier |
ISSN: | 0006-291X 1090-2104 |
Language: | English |
Subject (DDC): | 571: Physiology and related subjects 572: Biochemistry |
Abstract: | The rapid vascularisation of biomaterials and engineered tissue after implantation is a current unmet need. To this end, we explored the pharmacological option of inducing neovascularisation using compounds that inhibit hypoxia-induced factor-1α prolyl hydroxylase. This stabilises hypoxia inducible factor-1α and therefore de-repress the transcription of various angiogenic genes. In the quest for a small vertebrate model allowing for in vivo screening we exposed transgenic zebrafish embryos exhibiting fluorescent blood vessels to hydralazine hydrochloride and 2,4-pyridine dicarboxylic acid from 6 hpf to 72 hpf by immersion. Live observation of embryos revealed that the substances induced formation of ectopic blood vessels in the subintestinal vessel basket. We confirmed the HIF-stabilising effects biochemically in human fibroblasts and with an in vitro angiogenesis fibroblast/HUVEC co-culture model. Cross-inhibition of collagen prolyl hydroxylase was confirmed by reduced collagen secretion by fibroblasts and reduced collagen content of zebrafish embryos. |
URI: | https://digitalcollection.zhaw.ch/handle/11475/12206 |
Fulltext version: | Published version |
License (according to publishing contract): | Licence according to publishing contract |
Departement: | Life Sciences and Facility Management |
Organisational Unit: | Institute of Chemistry and Biotechnology (ICBT) |
Appears in collections: | Publikationen Life Sciences und Facility Management |
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