Novel use for polyvinylpyrrolidone as a macromolecular crowder for enhanced extracellular matrix deposition and cell proliferation

Rashid, Rafi; Lim, Natalie Sheng Jie; Chee, Stella Min Ling; Png, Si Ning; Wohland, Thorsten; Raghunath, Michael

doi:10.1089/ten.tec.2013.0733

Full metadata record

DC Field	Value	Language
dc.contributor.author	Rashid, Rafi	-
dc.contributor.author	Lim, Natalie Sheng Jie	-
dc.contributor.author	Chee, Stella Min Ling	-
dc.contributor.author	Png, Si Ning	-
dc.contributor.author	Wohland, Thorsten	-
dc.contributor.author	Raghunath, Michael	-
dc.date.accessioned	2018-10-26T14:42:36Z	-
dc.date.available	2018-10-26T14:42:36Z	-
dc.date.issued	2014	-
dc.identifier.issn	1937-3392	de_CH
dc.identifier.issn	1937-3384	de_CH
dc.identifier.uri	https://digitalcollection.zhaw.ch/handle/11475/12205	-
dc.description.abstract	Macromolecular crowding (MMC) is a biophysical effect that governs biochemical processes inside and outside of cells. Since standard cell culture media lack this effect, the physiological performance of differentiated and progenitor cells, including extracellular matrix (ECM) deposition, is impaired in vitro. To bring back physiological crowdedness to in vitro systems, we have previously introduced carbohydrate-based macromolecules to culture media and have achieved marked improvements with mixed MMC in terms of ECM deposition and differentiation of mesenchymal stem cells (MSCs). We show here that although this system is successful, it is limited, due to viscosity, to only 33% of the fractional volume occupancy (FVO) of full serum, which we calculated to have an FVO of approximately 54% v/v. We show here that full-serum FVO can be achieved using polyvinylpyrrolidone (PVP) 360 kDa. Under these conditions, ECM deposition in human fibroblasts and MSCs is on par, if not stronger than, with original MMC protocols using carbohydrates, but with a viscosity that is not significantly changed. In addition, we have found that the proliferation rate for bone marrow-derived MSCs and fibroblasts increases slightly in the presence of PVP360, similar to that observed with carbohydrate-based crowders. A palette of MMC compounds is now emerging that enables us to tune the crowdedness of culture media seamlessly from interstitial fluid (9% FVO), in which the majority of tissue cells might be based, to serum environments mimicking intravascular conditions. Despite identical FVO's, individual crowder size effects play a role and different cell types appear to have preferences in terms of FVO and the crowder that this is achieved with. However, in the quest of crowders that we have predicted to have a smoother regulatory approval path, PVP is a highly interesting compound, as it has been widely used in the medical and food industries and shows a novel promising use in cell culture and tissue engineering.	de_CH
dc.language.iso	en	de_CH
dc.publisher	Mary Ann Liebert	de_CH
dc.relation.ispartof	Tissue Engineering - Part C: Methods	de_CH
dc.rights	Licence according to publishing contract	de_CH
dc.subject.ddc	571: Physiologie und verwandte Themen	de_CH
dc.subject.ddc	572: Biochemie	de_CH
dc.title	Novel use for polyvinylpyrrolidone as a macromolecular crowder for enhanced extracellular matrix deposition and cell proliferation	de_CH
dc.type	Beitrag in wissenschaftlicher Zeitschrift	de_CH
dcterms.type	Text	de_CH
zhaw.departement	Life Sciences und Facility Management	de_CH
zhaw.organisationalunit	Institut für Chemie und Biotechnologie (ICBT)	de_CH
dc.identifier.doi	10.1089/ten.tec.2013.0733	de_CH
zhaw.funding.eu	No	de_CH
zhaw.issue	12	de_CH
zhaw.originated.zhaw	No	de_CH
zhaw.publication.status	publishedVersion	de_CH
zhaw.volume	20	de_CH
zhaw.publication.review	Peer review (Publikation)	de_CH
zhaw.webfeed	Metabolic Tissue Engineering	de_CH
Appears in collections:	Publikationen Life Sciences und Facility Management

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Rashid, R., Lim, N. S. J., Chee, S. M. L., Png, S. N., Wohland, T., & Raghunath, M. (2014). Novel use for polyvinylpyrrolidone as a macromolecular crowder for enhanced extracellular matrix deposition and cell proliferation. Tissue Engineering - Part C: Methods, 20(12). https://doi.org/10.1089/ten.tec.2013.0733

Rashid, R. et al. (2014) ‘Novel use for polyvinylpyrrolidone as a macromolecular crowder for enhanced extracellular matrix deposition and cell proliferation’, Tissue Engineering - Part C: Methods, 20(12). Available at: https://doi.org/10.1089/ten.tec.2013.0733.

R. Rashid, N. S. J. Lim, S. M. L. Chee, S. N. Png, T. Wohland, and M. Raghunath, “Novel use for polyvinylpyrrolidone as a macromolecular crowder for enhanced extracellular matrix deposition and cell proliferation,” Tissue Engineering - Part C: Methods, vol. 20, no. 12, 2014, doi: 10.1089/ten.tec.2013.0733.

RASHID, Rafi, Natalie Sheng Jie LIM, Stella Min Ling CHEE, Si Ning PNG, Thorsten WOHLAND und Michael RAGHUNATH, 2014. Novel use for polyvinylpyrrolidone as a macromolecular crowder for enhanced extracellular matrix deposition and cell proliferation. Tissue Engineering - Part C: Methods. 2014. Bd. 20, Nr. 12. DOI 10.1089/ten.tec.2013.0733

Rashid, Rafi, Natalie Sheng Jie Lim, Stella Min Ling Chee, Si Ning Png, Thorsten Wohland, and Michael Raghunath. 2014. “Novel Use for Polyvinylpyrrolidone as a Macromolecular Crowder for Enhanced Extracellular Matrix Deposition and Cell Proliferation.” Tissue Engineering - Part C: Methods 20 (12). https://doi.org/10.1089/ten.tec.2013.0733.

Rashid, Rafi, et al. “Novel Use for Polyvinylpyrrolidone as a Macromolecular Crowder for Enhanced Extracellular Matrix Deposition and Cell Proliferation.” Tissue Engineering - Part C: Methods, vol. 20, no. 12, 2014, https://doi.org/10.1089/ten.tec.2013.0733.