Accelerated development of supramolecular corneal stromal-like assemblies from corneal fibroblasts in the presence of macromolecular crowders

Kumar, Pramod; Satyam, Abhigyan; Fan, Xingliang; Rochev, Yury; Rodriguez, Brian J.; Gorelov, Alexander; Joshi, Lokesh; Raghunath, Michael; Pandit, Abhay; Zeugolis, Dimitrios I.

doi:10.1089/ten.TEC.2014.0387

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Accelerated development of supramolecular corneal stromal-like assemblies from corneal fibroblasts in the presence of macromolecular crowders
Authors:	Kumar, Pramod Satyam, Abhigyan Fan, Xingliang Rochev, Yury Rodriguez, Brian J. Gorelov, Alexander Joshi, Lokesh Raghunath, Michael Pandit, Abhay Zeugolis, Dimitrios I.
DOI:	10.1089/ten.TEC.2014.0387
Published in:	Tissue Engineering - Part C: Methods
Volume(Issue):	21
Issue:	7
Issue Date:	2015
Publisher / Ed. Institution:	Mary Ann Liebert
ISSN:	1937-3392 1937-3384
Language:	English
Subject (DDC):	571: Physiology and related subjects 610: Medicine and health
Abstract:	Tissue engineering by self-assembly uses the cells' secretome as a regeneration template and biological factory of trophic factors. Despite the several advantages that have been witnessed in preclinical and clinical settings, the major obstacle for wide acceptance of this technology remains the tardy extracellular matrix formation. In this study, we assessed the influence of macromolecular crowding (MMC)/excluding volume effect, a biophysical phenomenon that accelerates thermodynamic activities and biological processes by several orders of magnitude, in human corneal fibroblast (HCF) culture. Our data indicate that the addition of negatively charged galactose derivative (carrageenan) in HCF culture, even at 0.5% serum, increases by 12-fold tissue-specific matrix deposition, while maintaining physiological cell morphology and protein/gene expression. Gene analysis indicates that a glucose derivative (dextran sulfate) may drive corneal fibroblasts toward a myofibroblast lineage. Collectively, these results indicate that MMC may be suitable not only for clinical translation and commercialization of tissue engineering by self-assembly therapies, but also for the development of in vitro pathophysiology models.
URI:	https://digitalcollection.zhaw.ch/handle/11475/12204
Fulltext version:	Published version
License (according to publishing contract):	Licence according to publishing contract
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:	Publikationen Life Sciences und Facility Management

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Kumar, P., Satyam, A., Fan, X., Rochev, Y., Rodriguez, B. J., Gorelov, A., Joshi, L., Raghunath, M., Pandit, A., & Zeugolis, D. I. (2015). Accelerated development of supramolecular corneal stromal-like assemblies from corneal fibroblasts in the presence of macromolecular crowders. Tissue Engineering - Part C: Methods, 21(7). https://doi.org/10.1089/ten.TEC.2014.0387

Kumar, P. et al. (2015) ‘Accelerated development of supramolecular corneal stromal-like assemblies from corneal fibroblasts in the presence of macromolecular crowders’, Tissue Engineering - Part C: Methods, 21(7). Available at: https://doi.org/10.1089/ten.TEC.2014.0387.

P. Kumar et al., “Accelerated development of supramolecular corneal stromal-like assemblies from corneal fibroblasts in the presence of macromolecular crowders,” Tissue Engineering - Part C: Methods, vol. 21, no. 7, 2015, doi: 10.1089/ten.TEC.2014.0387.

KUMAR, Pramod, Abhigyan SATYAM, Xingliang FAN, Yury ROCHEV, Brian J. RODRIGUEZ, Alexander GORELOV, Lokesh JOSHI, Michael RAGHUNATH, Abhay PANDIT und Dimitrios I. ZEUGOLIS, 2015. Accelerated development of supramolecular corneal stromal-like assemblies from corneal fibroblasts in the presence of macromolecular crowders. Tissue Engineering - Part C: Methods. 2015. Bd. 21, Nr. 7. DOI 10.1089/ten.TEC.2014.0387

Kumar, Pramod, Abhigyan Satyam, Xingliang Fan, Yury Rochev, Brian J. Rodriguez, Alexander Gorelov, Lokesh Joshi, Michael Raghunath, Abhay Pandit, and Dimitrios I. Zeugolis. 2015. “Accelerated Development of Supramolecular Corneal Stromal-like Assemblies from Corneal Fibroblasts in the Presence of Macromolecular Crowders.” Tissue Engineering - Part C: Methods 21 (7). https://doi.org/10.1089/ten.TEC.2014.0387.

Kumar, Pramod, et al. “Accelerated Development of Supramolecular Corneal Stromal-like Assemblies from Corneal Fibroblasts in the Presence of Macromolecular Crowders.” Tissue Engineering - Part C: Methods, vol. 21, no. 7, 2015, https://doi.org/10.1089/ten.TEC.2014.0387.