Title: Simultaneous delivery of highly diverse bioactive compounds from blend electrospun fibers for skin wound healing
Authors : Peh, Priscilla
Lim, Natalie Sheng Jie
Blocki, Anna
Chee, Stella Min Ling
Park, Heyjin Chris
Liao, Susan
Chan, Casey
Raghunath, Michael
Published in : Bioconjugate chemistry
Volume(Issue) : 26
Issue : 7
Pages : 1348
Pages to: 1358
Publisher / Ed. Institution : American Chemical Society
Issue Date: 2015
License (according to publishing contract) : Licence according to publishing contract
Type of review: Peer review (Publication)
Language : English
Subject (DDC) : 572: Biochemistry
617: Surgery
Abstract: Blend emulsion electrospinning is widely perceived to destroy the bioactivity of proteins, and a blend emulsion of water-soluble and nonsoluble molecules is believed to be thermodynamically unstable to electrospin smoothly. Here we demonstrate a method to retain the bioactivity of disparate fragile biomolecules when electrospun. Using bovine serum albumin as a carrier protein; water-soluble vitamin C, fat soluble vitamin D3, steroid hormone hydrocortisone, peptide hormone insulin, thyroid hormone triiodothyronine (T3), and peptide epidermal growth factor (EGF) were simultaneously blend-spun into PLGA-collagen nanofibers. Upon release, vitamin C maintained the ability to facilitate Type I collagen secretion by fibroblasts, EGF stimulated skin fibroblast proliferation, and insulin potentiated adipogenic differentiation. Transgenic cell reporter assays confirmed the bioactivity of vitamin D3, T3, and hydrocortisone. These factors concertedly increased keratinocyte and fibroblast proliferation while maintaining keratinocyte basal state. This method presents an elegant solution to simultaneously deliver disparate bioactive biomolecules for wound healing applications.
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Chemistry and Biotechnology (ICBT)
Publication type: Article in scientific Journal
DOI : 10.1021/acs.bioconjchem.5b00123
ISSN: 1043-1802
1520-4812
URI: https://digitalcollection.zhaw.ch/handle/11475/12203
Appears in Collections:Publikationen Life Sciences und Facility Management

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