| Publikationstyp: | Beitrag in wissenschaftlicher Zeitschrift |
| Art der Begutachtung: | Peer review (Publikation) |
| Titel: | Tuning the architecture of 3D collagen hydrogels by physiological macromolecular crowding |
| Autor/-in: | Dewavrin, Jean-Yves Hamzavi, Nader Shim, V.P.W. Raghunath, Michael |
| DOI: | 10.1016/j.actbio.2014.06.006 |
| Erschienen in: | Acta Biomaterialia |
| Band(Heft): | 10 |
| Heft: | 10 |
| Seite(n): | 4351 |
| Seiten bis: | 4359 |
| Erscheinungsdatum: | 2014 |
| Verlag / Hrsg. Institution: | Elsevier |
| ISSN: | 1742-7061 1878-7568 |
| Sprache: | Englisch |
| Fachgebiet (DDC): | 571: Physiologie und verwandte Themen 572: Biochemie |
| Zusammenfassung: | Macromolecular crowding is an optimal physiological feature in intracellular and extracellular spaces, and results from a variety of macromolecules occupying space and contributing to a fractional volume occupancy. Here, we show that soft collagen hydrogels assembled in nature-inspired crowded conditions feature enhanced biophysical properties. We demonstrate that crowding tunes the rate of collagen nucleation and fiber growth, affecting fiber diameter and organization. Adjustments of crowding levels during collagen assembly tune the gel pore size, protein permeability, transparency and resistance to enzymatic degradation. Furthermore, gels assembled in crowded conditions are twice as resistant to mechanical stress as the controls, inducing a 70% boost of proliferation of stem cells cultured on tuned hydrogels. Emulating the crowdedness of interstitial fluids therefore represents a way to optimize the properties of soft collagen gels, with promising applications in soft biomaterials design. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/12200 |
| Volltext Version: | Publizierte Version |
| Lizenz (gemäss Verlagsvertrag): | Lizenz gemäss Verlagsvertrag |
| Departement: | Life Sciences und Facility Management |
| Organisationseinheit: | Institut für Chemie und Biotechnologie (ICBT) |
| Enthalten in den Sammlungen: | Publikationen Life Sciences und Facility Management |
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Dewavrin, J.-Y., Hamzavi, N., Shim, V. P. W., & Raghunath, M. (2014). Tuning the architecture of 3D collagen hydrogels by physiological macromolecular crowding. Acta Biomaterialia, 10(10), 4351–4359. https://doi.org/10.1016/j.actbio.2014.06.006
Dewavrin, J.-Y. et al. (2014) ‘Tuning the architecture of 3D collagen hydrogels by physiological macromolecular crowding’, Acta Biomaterialia, 10(10), pp. 4351–4359. Available at: https://doi.org/10.1016/j.actbio.2014.06.006.
J.-Y. Dewavrin, N. Hamzavi, V. P. W. Shim, and M. Raghunath, “Tuning the architecture of 3D collagen hydrogels by physiological macromolecular crowding,” Acta Biomaterialia, vol. 10, no. 10, pp. 4351–4359, 2014, doi: 10.1016/j.actbio.2014.06.006.
DEWAVRIN, Jean-Yves, Nader HAMZAVI, V.P.W. SHIM und Michael RAGHUNATH, 2014. Tuning the architecture of 3D collagen hydrogels by physiological macromolecular crowding. Acta Biomaterialia. 2014. Bd. 10, Nr. 10, S. 4351–4359. DOI 10.1016/j.actbio.2014.06.006
Dewavrin, Jean-Yves, Nader Hamzavi, V.P.W. Shim, and Michael Raghunath. 2014. “Tuning the Architecture of 3D Collagen Hydrogels by Physiological Macromolecular Crowding.” Acta Biomaterialia 10 (10): 4351–59. https://doi.org/10.1016/j.actbio.2014.06.006.
Dewavrin, Jean-Yves, et al. “Tuning the Architecture of 3D Collagen Hydrogels by Physiological Macromolecular Crowding.” Acta Biomaterialia, vol. 10, no. 10, 2014, pp. 4351–59, https://doi.org/10.1016/j.actbio.2014.06.006.
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