Title: Tuning the architecture of 3D collagen hydrogels by physiological macromolecular crowding
Authors : Dewavrin, Jean-Yves
Hamzavi, Nader
Shim, V.P.W.
Raghunath, Michael
Published in : Acta biomaterialia
Volume(Issue) : 10
Issue : 10
Pages : 4351
Pages to: 4359
Publisher / Ed. Institution : Elsevier
Issue Date: 2014
License (according to publishing contract) : Licence according to publishing contract
Type of review: Peer review (Publication)
Language : English
Subject (DDC) : 571: Physiology and related subjects
572: Biochemistry
Abstract: Macromolecular crowding is an optimal physiological feature in intracellular and extracellular spaces, and results from a variety of macromolecules occupying space and contributing to a fractional volume occupancy. Here, we show that soft collagen hydrogels assembled in nature-inspired crowded conditions feature enhanced biophysical properties. We demonstrate that crowding tunes the rate of collagen nucleation and fiber growth, affecting fiber diameter and organization. Adjustments of crowding levels during collagen assembly tune the gel pore size, protein permeability, transparency and resistance to enzymatic degradation. Furthermore, gels assembled in crowded conditions are twice as resistant to mechanical stress as the controls, inducing a 70% boost of proliferation of stem cells cultured on tuned hydrogels. Emulating the crowdedness of interstitial fluids therefore represents a way to optimize the properties of soft collagen gels, with promising applications in soft biomaterials design.
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Chemistry and Biotechnology (ICBT)
Publication type: Article in scientific Journal
DOI : 10.1016/j.actbio.2014.06.006
ISSN: 1742-7061
URI: https://digitalcollection.zhaw.ch/handle/11475/12200
Appears in Collections:Publikationen Life Sciences und Facility Management

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