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dc.contributor.authorKumar, Pramod-
dc.contributor.authorSatyam, Abhigyan-
dc.contributor.authorFan, Xingliang-
dc.contributor.authorCollin, Estelle-
dc.contributor.authorRochev, Yury-
dc.contributor.authorRodriguez, Brian J.-
dc.contributor.authorGorelov, Alexander-
dc.contributor.authorDillon, Simon-
dc.contributor.authorJoshi, Lokesh-
dc.contributor.authorRaghunath, Michael-
dc.contributor.authorPandit, Abhay-
dc.contributor.authorZeugolis, Dimitrios I.-
dc.description.abstractTherapeutic strategies based on the principles of tissue engineering by self-assembly put forward the notion that functional regeneration can be achieved by utilising the inherent capacity of cells to create highly sophisticated supramolecular assemblies. However, in dilute ex-vivo microenvironments, prolonged culture time is required to develop an extracellular matrix-rich implantable device. Herein, we assessed the influence of macromolecular crowding, a biophysical phenomenon that regulates intra- and extra-cellular activities in multicellular organisms, in human corneal fibroblast culture. In the presence of macromolecules, abundant extracellular matrix deposition was evidenced as fast as 48 h in culture, even at low serum concentration. Temperature responsive copolymers allowed the detachment of dense and cohesive supramolecularly assembled living substitutes within 6 days in culture. Morphological, histological, gene and protein analysis assays demonstrated maintenance of tissue-specific function. Macromolecular crowding opens new avenues for a more rational design in engineering of clinically relevant tissue modules in vitro.de_CH
dc.publisherNature Publishing Groupde_CH
dc.relation.ispartofScientific Reportsde_CH
dc.subject.ddc571: Physiologie und verwandte Themende_CH
dc.subject.ddc572: Biochemiede_CH
dc.titleMacromolecularly crowded in vitro microenvironments accelerate the production of extracellular matrix-rich supramolecular assembliesde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.webfeed3D Gewebe und Biofabrikationde_CH
Appears in Collections:Publikationen Life Sciences und Facility Management

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