Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-4754
Publication type: Article in scientific journal
Type of review: Peer review (publication)
Title: Macromolecularly crowded in vitro microenvironments accelerate the production of extracellular matrix-rich supramolecular assemblies
Authors : Kumar, Pramod
Satyam, Abhigyan
Fan, Xingliang
Collin, Estelle
Rochev, Yury
Rodriguez, Brian J.
Gorelov, Alexander
Dillon, Simon
Joshi, Lokesh
Raghunath, Michael
Pandit, Abhay
Zeugolis, Dimitrios I.
DOI : 10.21256/zhaw-4754
10.1038/srep08729
Published in : Scientific Reports
Volume(Issue) : 5
Issue : 8729
Issue Date: 2015
Publisher / Ed. Institution : Nature Publishing Group
ISSN: 2045-2322
Language : English
Subject (DDC) : 571: Physiology and related subjects
572: Biochemistry
Abstract: Therapeutic strategies based on the principles of tissue engineering by self-assembly put forward the notion that functional regeneration can be achieved by utilising the inherent capacity of cells to create highly sophisticated supramolecular assemblies. However, in dilute ex-vivo microenvironments, prolonged culture time is required to develop an extracellular matrix-rich implantable device. Herein, we assessed the influence of macromolecular crowding, a biophysical phenomenon that regulates intra- and extra-cellular activities in multicellular organisms, in human corneal fibroblast culture. In the presence of macromolecules, abundant extracellular matrix deposition was evidenced as fast as 48 h in culture, even at low serum concentration. Temperature responsive copolymers allowed the detachment of dense and cohesive supramolecularly assembled living substitutes within 6 days in culture. Morphological, histological, gene and protein analysis assays demonstrated maintenance of tissue-specific function. Macromolecular crowding opens new avenues for a more rational design in engineering of clinically relevant tissue modules in vitro.
URI: https://digitalcollection.zhaw.ch/handle/11475/12199
Fulltext version : Published version
License (according to publishing contract) : CC BY 4.0: Attribution 4.0 International
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Chemistry and Biotechnology (ICBT)
Appears in Collections:Publikationen Life Sciences und Facility Management

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