Macromolecularly crowded in vitro microenvironments accelerate the production of extracellular matrix-rich supramolecular assemblies

Kumar, Pramod; Satyam, Abhigyan; Fan, Xingliang; Collin, Estelle; Rochev, Yury; Rodriguez, Brian J.; Gorelov, Alexander; Dillon, Simon; Joshi, Lokesh; Raghunath, Michael; Pandit, Abhay; Zeugolis, Dimitrios I.

doi:10.21256/zhaw-4754

Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-4754

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Macromolecularly crowded in vitro microenvironments accelerate the production of extracellular matrix-rich supramolecular assemblies
Authors:	Kumar, Pramod Satyam, Abhigyan Fan, Xingliang Collin, Estelle Rochev, Yury Rodriguez, Brian J. Gorelov, Alexander Dillon, Simon Joshi, Lokesh Raghunath, Michael Pandit, Abhay Zeugolis, Dimitrios I.
DOI:	10.21256/zhaw-4754 10.1038/srep08729
Published in:	Scientific Reports
Volume(Issue):	5
Issue:	8729
Issue Date:	2015
Publisher / Ed. Institution:	Nature Publishing Group
ISSN:	2045-2322
Language:	English
Subject (DDC):	571: Physiology and related subjects 572: Biochemistry
Abstract:	Therapeutic strategies based on the principles of tissue engineering by self-assembly put forward the notion that functional regeneration can be achieved by utilising the inherent capacity of cells to create highly sophisticated supramolecular assemblies. However, in dilute ex-vivo microenvironments, prolonged culture time is required to develop an extracellular matrix-rich implantable device. Herein, we assessed the influence of macromolecular crowding, a biophysical phenomenon that regulates intra- and extra-cellular activities in multicellular organisms, in human corneal fibroblast culture. In the presence of macromolecules, abundant extracellular matrix deposition was evidenced as fast as 48 h in culture, even at low serum concentration. Temperature responsive copolymers allowed the detachment of dense and cohesive supramolecularly assembled living substitutes within 6 days in culture. Morphological, histological, gene and protein analysis assays demonstrated maintenance of tissue-specific function. Macromolecular crowding opens new avenues for a more rational design in engineering of clinically relevant tissue modules in vitro.
URI:	https://digitalcollection.zhaw.ch/handle/11475/12199
Fulltext version:	Published version
License (according to publishing contract):	CC BY 4.0: Attribution 4.0 International
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:	Publikationen Life Sciences und Facility Management

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Kumar, P., Satyam, A., Fan, X., Collin, E., Rochev, Y., Rodriguez, B. J., Gorelov, A., Dillon, S., Joshi, L., Raghunath, M., Pandit, A., & Zeugolis, D. I. (2015). Macromolecularly crowded in vitro microenvironments accelerate the production of extracellular matrix-rich supramolecular assemblies. Scientific Reports, 5(8729). https://doi.org/10.21256/zhaw-4754

Kumar, P. et al. (2015) ‘Macromolecularly crowded in vitro microenvironments accelerate the production of extracellular matrix-rich supramolecular assemblies’, Scientific Reports, 5(8729). Available at: https://doi.org/10.21256/zhaw-4754.

P. Kumar et al., “Macromolecularly crowded in vitro microenvironments accelerate the production of extracellular matrix-rich supramolecular assemblies,” Scientific Reports, vol. 5, no. 8729, 2015, doi: 10.21256/zhaw-4754.

KUMAR, Pramod, Abhigyan SATYAM, Xingliang FAN, Estelle COLLIN, Yury ROCHEV, Brian J. RODRIGUEZ, Alexander GORELOV, Simon DILLON, Lokesh JOSHI, Michael RAGHUNATH, Abhay PANDIT und Dimitrios I. ZEUGOLIS, 2015. Macromolecularly crowded in vitro microenvironments accelerate the production of extracellular matrix-rich supramolecular assemblies. Scientific Reports. 2015. Bd. 5, Nr. 8729. DOI 10.21256/zhaw-4754

Kumar, Pramod, Abhigyan Satyam, Xingliang Fan, Estelle Collin, Yury Rochev, Brian J. Rodriguez, Alexander Gorelov, et al. 2015. “Macromolecularly Crowded in Vitro Microenvironments Accelerate the Production of Extracellular Matrix-Rich Supramolecular Assemblies.” Scientific Reports 5 (8729). https://doi.org/10.21256/zhaw-4754.

Kumar, Pramod, et al. “Macromolecularly Crowded in Vitro Microenvironments Accelerate the Production of Extracellular Matrix-Rich Supramolecular Assemblies.” Scientific Reports, vol. 5, no. 8729, 2015, https://doi.org/10.21256/zhaw-4754.