Publication type: | Article in scientific journal |
Type of review: | Peer review (publication) |
Title: | RGB‐Color intensiometric indicators to visualize spatiotemporal dynamics of ATP in single cells |
Authors: | Arai, Satoshi Kriszt, Rókus Harada, Kazuki Looi, Liang‐Sheng Matsuda, Shogo Wongso, Devina Suo, Satoshi Ishiura, Shoichi Tseng, Yu‐Hua Raghunath, Michael Ito, Toshiro Tsuboi, Takashi Kitaguchi, Tetsuya |
DOI: | 10.1002/anie.201804304 |
Published in: | Angewandte Chemie: International Edition |
Volume(Issue): | 57 |
Issue: | 34 |
Page(s): | 10873 |
Pages to: | 10878 |
Issue Date: | 2018 |
Publisher / Ed. Institution: | Wiley |
ISSN: | 1433-7851 0570-0833 |
Language: | English |
Subject (DDC): | 572: Biochemistry |
Abstract: | Adenosine triphosphate (ATP) provides energy for the regulation of multiple cellular processes in living organisms. Capturing the spatiotemporal dynamics of ATP in single cells is fundamental to our understanding of the mechanisms underlying cellular energy metabolism. However, it has remained challenging to visualize the dynamics of ATP in and between distinct intracellular organelles and its interplay with other signaling molecules. Using single fluorescent proteins, multicolor ATP indicators were developed, enabling the simultaneous visualization of subcellular ATP dynamics in the cytoplasm and mitochondria of cells derived from mammals, plants, and worms. Furthermore, in combination with additional fluorescent indicators, the dynamic interplay of ATP, cAMP, and Ca2+ could be visualized in activated brown adipocyte. This set of indicator tools will facilitate future research into energy metabolism. |
URI: | https://digitalcollection.zhaw.ch/handle/11475/12102 |
Fulltext version: | Published version |
License (according to publishing contract): | Licence according to publishing contract |
Departement: | Life Sciences and Facility Management |
Organisational Unit: | Institute of Chemistry and Biotechnology (ICBT) |
Appears in collections: | Publikationen Life Sciences und Facility Management |
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Arai, S., Kriszt, R., Harada, K., Looi, L.-S., Matsuda, S., Wongso, D., Suo, S., Ishiura, S., Tseng, Y.-H., Raghunath, M., Ito, T., Tsuboi, T., & Kitaguchi, T. (2018). RGB‐Color intensiometric indicators to visualize spatiotemporal dynamics of ATP in single cells. Angewandte Chemie: International Edition, 57(34), 10873–10878. https://doi.org/10.1002/anie.201804304
Arai, S. et al. (2018) ‘RGB‐Color intensiometric indicators to visualize spatiotemporal dynamics of ATP in single cells’, Angewandte Chemie: International Edition, 57(34), pp. 10873–10878. Available at: https://doi.org/10.1002/anie.201804304.
S. Arai et al., “RGB‐Color intensiometric indicators to visualize spatiotemporal dynamics of ATP in single cells,” Angewandte Chemie: International Edition, vol. 57, no. 34, pp. 10873–10878, 2018, doi: 10.1002/anie.201804304.
ARAI, Satoshi, Rókus KRISZT, Kazuki HARADA, Liang‐Sheng LOOI, Shogo MATSUDA, Devina WONGSO, Satoshi SUO, Shoichi ISHIURA, Yu‐Hua TSENG, Michael RAGHUNATH, Toshiro ITO, Takashi TSUBOI und Tetsuya KITAGUCHI, 2018. RGB‐Color intensiometric indicators to visualize spatiotemporal dynamics of ATP in single cells. Angewandte Chemie: International Edition. 2018. Bd. 57, Nr. 34, S. 10873–10878. DOI 10.1002/anie.201804304
Arai, Satoshi, Rókus Kriszt, Kazuki Harada, Liang‐Sheng Looi, Shogo Matsuda, Devina Wongso, Satoshi Suo, et al. 2018. “RGB‐Color Intensiometric Indicators to Visualize Spatiotemporal Dynamics of ATP in Single Cells.” Angewandte Chemie: International Edition 57 (34): 10873–78. https://doi.org/10.1002/anie.201804304.
Arai, Satoshi, et al. “RGB‐Color Intensiometric Indicators to Visualize Spatiotemporal Dynamics of ATP in Single Cells.” Angewandte Chemie: International Edition, vol. 57, no. 34, 2018, pp. 10873–78, https://doi.org/10.1002/anie.201804304.
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