Title: RGB‐Color intensiometric indicators to visualize spatiotemporal dynamics of ATP in single cells
Authors : Arai, Satoshi
Kriszt, Rókus
Harada, Kazuki
Looi, Liang‐Sheng
Matsuda, Shogo
Wongso, Devina
Suo, Satoshi
Ishiura, Shoichi
Tseng, Yu‐Hua
Raghunath, Michael
Ito, Toshiro
Tsuboi, Takashi
Kitaguchi, Tetsuya
Published in : Angewandte Chemie : international edition
Volume(Issue) : 57
Issue : 34
Pages : 10873
Pages to: 10878
Publisher / Ed. Institution : Wiley
Issue Date: 2018
License (according to publishing contract) : Licence according to publishing contract
Type of review: Peer review (Publication)
Language : English
Subject (DDC) : 572: Biochemistry
Abstract: Adenosine triphosphate (ATP) provides energy for the regulation of multiple cellular processes in living organisms. Capturing the spatiotemporal dynamics of ATP in single cells is fundamental to our understanding of the mechanisms underlying cellular energy metabolism. However, it has remained challenging to visualize the dynamics of ATP in and between distinct intracellular organelles and its interplay with other signaling molecules. Using single fluorescent proteins, multicolor ATP indicators were developed, enabling the simultaneous visualization of subcellular ATP dynamics in the cytoplasm and mitochondria of cells derived from mammals, plants, and worms. Furthermore, in combination with additional fluorescent indicators, the dynamic interplay of ATP, cAMP, and Ca2+ could be visualized in activated brown adipocyte. This set of indicator tools will facilitate future research into energy metabolism.
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Chemistry and Biotechnology (ICBT)
Publication type: Article in scientific Journal
DOI : 10.1002/anie.201804304
ISSN: 1433-7851
0570-0833
URI: https://digitalcollection.zhaw.ch/handle/11475/12102
Appears in Collections:Publikationen Life Sciences und Facility Management

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