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https://doi.org/10.21256/zhaw-4839Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Hou, Yanyan | - |
| dc.contributor.author | Kitaguchi, Tetsuya | - |
| dc.contributor.author | Kriszt, Rókus | - |
| dc.contributor.author | Tseng, Yu-Hua | - |
| dc.contributor.author | Raghunath, Michael | - |
| dc.contributor.author | Suzuki, Madoka | - |
| dc.date.accessioned | 2018-10-24T09:39:09Z | - |
| dc.date.available | 2018-10-24T09:39:09Z | - |
| dc.date.issued | 2017 | - |
| dc.identifier.issn | 2212-8778 | de_CH |
| dc.identifier.uri | https://digitalcollection.zhaw.ch/handle/11475/12099 | - |
| dc.description.abstract | Objective: Brown adipocytes (BAs) are endowed with a high metabolic capacity for energy expenditure due to their high mitochondria content. While mitochondrial pH is dynamically regulated in response to stimulation and, in return, affects various metabolic processes, how mitochondrial pH is regulated during adrenergic stimulation-induced thermogenesis is unknown. We aimed to reveal the spatial and temporal dynamics of mitochondrial pH in stimulated BAs and the mechanisms behind the dynamic pH changes. Methods: A mitochondrial targeted pH-sensitive protein, mito-pHluorin, was constructed and transfected to BAs. Transfected BAs were stimulated by an adrenergic agonist, isoproterenol. The pH changes in mitochondria were characterized by dual-color imaging with indicators that monitor mitochondrial membrane potential and heat production. The mechanisms of pH changes were studied by examining the involvement of electron transport chain (ETC) activity and Ca2+ profiles in mitochondria and the intracellular Ca2+ store, the endoplasmic reticulum (ER). Results: A triphasic mitochondrial pH change in BAs upon adrenergic stimulation was revealed. In comparison to a thermosensitive dye, we reveal that phases 1 and 2 of the pH increase precede thermogenesis, while phase 3, characterized by a pH decrease, occurs during thermogenesis. The mechanism of pH increase is partially related to ETC. In addition, the pH increase occurs concurrently with an increase in mitochondrial Ca2+. This Ca2+ increase is contributed to by an influx from the ER, and it is further involved in mitochondrial pH regulation. Conclusions: We demonstrate that an increase in mitochondrial pH is implicated as an early event in adrenergically stimulated BAs. We further suggest that this pH increase may play a role in the potentiation of thermogenesis. | de_CH |
| dc.language.iso | en | de_CH |
| dc.publisher | Elsevier | de_CH |
| dc.relation.ispartof | Molecular Metabolism | de_CH |
| dc.rights | http://creativecommons.org/licenses/by/4.0/ | de_CH |
| dc.subject.ddc | 572: Biochemie | de_CH |
| dc.subject.ddc | 610: Medizin und Gesundheit | de_CH |
| dc.title | Ca2+-associated triphasic pH changes in mitochondria during brown adipocyte activation | de_CH |
| dc.type | Beitrag in wissenschaftlicher Zeitschrift | de_CH |
| dcterms.type | Text | de_CH |
| zhaw.departement | Life Sciences und Facility Management | de_CH |
| zhaw.organisationalunit | Institut für Chemie und Biotechnologie (ICBT) | de_CH |
| dc.identifier.doi | 10.21256/zhaw-4839 | - |
| dc.identifier.doi | 10.1016/j.molmet.2017.05.013 | de_CH |
| zhaw.funding.eu | No | de_CH |
| zhaw.issue | 8 | de_CH |
| zhaw.originated.zhaw | Yes | de_CH |
| zhaw.pages.end | 808 | de_CH |
| zhaw.pages.start | 797 | de_CH |
| zhaw.publication.status | publishedVersion | de_CH |
| zhaw.volume | 6 | de_CH |
| zhaw.publication.review | Peer review (Publikation) | de_CH |
| zhaw.webfeed | Metabolic Tissue Engineering | de_CH |
| Appears in collections: | Publikationen Life Sciences und Facility Management | |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| 2017_Hou_Ca2_associated_triphasic_pH_changes_in_mitochondria.pdf | 6.18 MB | Adobe PDF |  View/Open |
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Hou, Y., Kitaguchi, T., Kriszt, R., Tseng, Y.-H., Raghunath, M., & Suzuki, M. (2017). Ca2+-associated triphasic pH changes in mitochondria during brown adipocyte activation. Molecular Metabolism, 6(8), 797–808. https://doi.org/10.21256/zhaw-4839
Hou, Y. et al. (2017) ‘Ca2+-associated triphasic pH changes in mitochondria during brown adipocyte activation’, Molecular Metabolism, 6(8), pp. 797–808. Available at: https://doi.org/10.21256/zhaw-4839.
Y. Hou, T. Kitaguchi, R. Kriszt, Y.-H. Tseng, M. Raghunath, and M. Suzuki, “Ca2+-associated triphasic pH changes in mitochondria during brown adipocyte activation,” Molecular Metabolism, vol. 6, no. 8, pp. 797–808, 2017, doi: 10.21256/zhaw-4839.
HOU, Yanyan, Tetsuya KITAGUCHI, Rókus KRISZT, Yu-Hua TSENG, Michael RAGHUNATH und Madoka SUZUKI, 2017. Ca2+-associated triphasic pH changes in mitochondria during brown adipocyte activation. Molecular Metabolism. 2017. Bd. 6, Nr. 8, S. 797–808. DOI 10.21256/zhaw-4839
Hou, Yanyan, Tetsuya Kitaguchi, Rókus Kriszt, Yu-Hua Tseng, Michael Raghunath, and Madoka Suzuki. 2017. “Ca2+-Associated Triphasic pH Changes in Mitochondria during Brown Adipocyte Activation.” Molecular Metabolism 6 (8): 797–808. https://doi.org/10.21256/zhaw-4839.
Hou, Yanyan, et al. “Ca2+-Associated Triphasic pH Changes in Mitochondria during Brown Adipocyte Activation.” Molecular Metabolism, vol. 6, no. 8, 2017, pp. 797–808, https://doi.org/10.21256/zhaw-4839.
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