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dc.contributor.authorBlocki, Anna-
dc.contributor.authorWang, Yingting-
dc.contributor.authorKoch, Maria-
dc.contributor.authorPeh, Priscilla-
dc.contributor.authorBeyer, Sebastian-
dc.contributor.authorLaw, Ping-
dc.contributor.authorHui, James-
dc.contributor.authorRaghunath, Michael-
dc.date.accessioned2018-10-23T14:34:05Z-
dc.date.available2018-10-23T14:34:05Z-
dc.date.issued2013-
dc.identifier.issn1557-8534de_CH
dc.identifier.issn1547-3287de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/12072-
dc.description.abstractPericytes play a crucial role in angiogenesis and vascular maintenance. They can be readily identified in vivo and isolated as CD146+CD34-cells from various tissues. Whether these and other markers reliably identify pericytes in vitro is unclear. CD146+CD34− selected cells exhibit multilineage potential. Thus, their perivascular location might represent a stem cell niche. This has spurred assumptions that not only all pericytes are mesenchymal stromal cells (MSCs), but also that all MSCs can act as pericytes. Considering this hypothesis, we developed functional assays by confronting test cells with endothelial cultures based on matrigel assay, spheroid sprouting, and cord formation. We calibrated these assays first with commercial cell lines [CD146+CD34-placenta-derived pericytes (Pl-Prc), bone marrow (bm)MSCs and fibroblasts]. We then functionally compared the angiogenic abilities of CD146+CD34-selected bmMSCs with CD146-selected bmMSCs from fresh human bm aspirates. We show here that only CD146+CD3-selected Pl-Prc and CD146+CD34-selected bmMSCs maintain endothelial tubular networks on matrigel and improve endothelial sprout morphology. CD146-selected bmMSCs neither showed these abilities, nor did they attain pericyte function despite progressive CD146 expression once passaged. Thus, cell culture conditions appear to influence expression of this and other reported pericyte markers significantly without correlation to function. The newly developed assays, therefore, promise to close a gap in the in vitro identification of pericytes via function. Indeed, our functional data suggest that pericytes represent a subpopulation of MSCs in bm with a specialized role in vascular biology. However, these functions are not inherent to all MSCs.de_CH
dc.language.isoende_CH
dc.publisherLiebertde_CH
dc.relation.ispartofStem Cells and Developmentde_CH
dc.rightsLicence according to publishing contractde_CH
dc.subject.ddc570: Biologiede_CH
dc.subject.ddc610: Medizin und Gesundheitde_CH
dc.titleNot all MSCs can act as pericytes : functional In vitro assays to distinguish pericytes from other mesenchymal stem cells in angiogenesisde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
dc.identifier.doi10.1089/scd.2012.0415de_CH
zhaw.funding.euNode_CH
zhaw.issue17de_CH
zhaw.originated.zhawNode_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume22de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.webfeedMetabolic Tissue Engineeringde_CH
Appears in collections:Publikationen Life Sciences und Facility Management

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Blocki, A., Wang, Y., Koch, M., Peh, P., Beyer, S., Law, P., Hui, J., & Raghunath, M. (2013). Not all MSCs can act as pericytes : functional In vitro assays to distinguish pericytes from other mesenchymal stem cells in angiogenesis. Stem Cells and Development, 22(17). https://doi.org/10.1089/scd.2012.0415
Blocki, A. et al. (2013) ‘Not all MSCs can act as pericytes : functional In vitro assays to distinguish pericytes from other mesenchymal stem cells in angiogenesis’, Stem Cells and Development, 22(17). Available at: https://doi.org/10.1089/scd.2012.0415.
A. Blocki et al., “Not all MSCs can act as pericytes : functional In vitro assays to distinguish pericytes from other mesenchymal stem cells in angiogenesis,” Stem Cells and Development, vol. 22, no. 17, 2013, doi: 10.1089/scd.2012.0415.
BLOCKI, Anna, Yingting WANG, Maria KOCH, Priscilla PEH, Sebastian BEYER, Ping LAW, James HUI und Michael RAGHUNATH, 2013. Not all MSCs can act as pericytes : functional In vitro assays to distinguish pericytes from other mesenchymal stem cells in angiogenesis. Stem Cells and Development. 2013. Bd. 22, Nr. 17. DOI 10.1089/scd.2012.0415
Blocki, Anna, Yingting Wang, Maria Koch, Priscilla Peh, Sebastian Beyer, Ping Law, James Hui, and Michael Raghunath. 2013. “Not All MSCs Can Act as Pericytes : Functional in Vitro Assays to Distinguish Pericytes from Other Mesenchymal Stem Cells in Angiogenesis.” Stem Cells and Development 22 (17). https://doi.org/10.1089/scd.2012.0415.
Blocki, Anna, et al. “Not All MSCs Can Act as Pericytes : Functional in Vitro Assays to Distinguish Pericytes from Other Mesenchymal Stem Cells in Angiogenesis.” Stem Cells and Development, vol. 22, no. 17, 2013, https://doi.org/10.1089/scd.2012.0415.


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