Title: Not all MSCs can act as pericytes : functional In vitro assays to distinguish pericytes from other mesenchymal stem cells in angiogenesis
Authors : Blocki, Anna
Wang, Yingting
Koch, Maria
Peh, Priscilla
Beyer, Sebastian
Law, Ping
Hui, James
Raghunath, Michael
Published in : Stem Cells and Development
Volume(Issue) : 22
Issue : 17
Publisher / Ed. Institution : Liebert
Issue Date: 2013
License (according to publishing contract) : Licence according to publishing contract
Type of review: Peer review (publication)
Language : English
Subject (DDC) : 570: Biology
610: Medicine and health
Abstract: Pericytes play a crucial role in angiogenesis and vascular maintenance. They can be readily identified in vivo and isolated as CD146+CD34-cells from various tissues. Whether these and other markers reliably identify pericytes in vitro is unclear. CD146+CD34− selected cells exhibit multilineage potential. Thus, their perivascular location might represent a stem cell niche. This has spurred assumptions that not only all pericytes are mesenchymal stromal cells (MSCs), but also that all MSCs can act as pericytes. Considering this hypothesis, we developed functional assays by confronting test cells with endothelial cultures based on matrigel assay, spheroid sprouting, and cord formation. We calibrated these assays first with commercial cell lines [CD146+CD34-placenta-derived pericytes (Pl-Prc), bone marrow (bm)MSCs and fibroblasts]. We then functionally compared the angiogenic abilities of CD146+CD34-selected bmMSCs with CD146-selected bmMSCs from fresh human bm aspirates. We show here that only CD146+CD3-selected Pl-Prc and CD146+CD34-selected bmMSCs maintain endothelial tubular networks on matrigel and improve endothelial sprout morphology. CD146-selected bmMSCs neither showed these abilities, nor did they attain pericyte function despite progressive CD146 expression once passaged. Thus, cell culture conditions appear to influence expression of this and other reported pericyte markers significantly without correlation to function. The newly developed assays, therefore, promise to close a gap in the in vitro identification of pericytes via function. Indeed, our functional data suggest that pericytes represent a subpopulation of MSCs in bm with a specialized role in vascular biology. However, these functions are not inherent to all MSCs.
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Chemistry and Biotechnology (ICBT)
Publication type: Article in scientific journal
DOI : 10.1089/scd.2012.0415
ISSN: 1557-8534
URI: https://digitalcollection.zhaw.ch/handle/11475/12072
Appears in Collections:Publikationen Life Sciences und Facility Management

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