Title: Telomere length analysis of human mesenchymal stem cells by quantitative PCR
Authors : Samsonraj, Rebekah M
Raghunath, Michael
Hui, James H
Ling, Ling
Nurcombe, Victor
Cool, Simon M
Published in : Gene
Volume(Issue) : 519
Issue : 2
Pages : 348
Pages to: 355
Publisher / Ed. Institution : Elsevier
Issue Date: 1-May-2013
License (according to publishing contract) : Licence according to publishing contract
Type of review: Peer review (Publication)
Language : English
Subjects : Southern blotting; Cell proliferation; Cell survival; Human chromosomes; DNA; Gene dosage; Gene expression; Fluorescence in situ hybridization; Mesenchymal stromal cells; Real-time polymerase chain reaction; Telomerase; Telomere shortening
Subject (DDC) : 570: Biology
610: Medicine and health
Abstract: Human mesenchymal stem cells (hMSCs) have attracted much attention for tissue repair and wound healing because of their self-renewal capacity and multipotentiality. In order to mediate an effective therapy, substantial numbers of cells are required, which necessitates extensive sub-culturing and expansion of hMSCs. Throughout ex vivo expansion, the cells undergo telomere shortening, and critically short telomeres can trigger loss of cell viability. Telomeres are nucleoprotein structures that cap the ends of chromosomes, and serve to protect the DNA from the degradation which occurs due to the end-replication problem in all eukaryotes. As hMSCs have only a finite ability for self-renewal like most somatic cells, assaying for telomere length in hMSCs provides critical information on the replicative capacity of the cells, an important criterion in the selection of hMSCs for therapy. Telomere length is generally quantified by Southern blotting and fluorescence in situ hybridization, and more recently by PCR-based methods. Here we describe the quantification of hMSC telomere length by real-time PCR; our results demonstrate the effect of telomere shortening on the proliferation and clonogenicity of hMSCs. Thus, this assay constitutes a useful tool for the determination of relative telomere length in hMSCs.
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Chemistry and Biotechnology (ICBT)
Publication type: Article in scientific Journal
DOI : 10.1016/j.gene.2013.01.039
ISSN: 1879-0038
0378-1119
URI: https://digitalcollection.zhaw.ch/handle/11475/12071
Appears in Collections:Publikationen Life Sciences und Facility Management

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