|Publication type:||Article in scientific journal|
|Type of review:||Peer review (publication)|
|Title:||Cloning, sequence analysis and expression of the F1F0- ATPase beta-subunit from wine lactic acid bacteria|
|Published in:||Systematic and Applied Microbiology|
|Publisher / Ed. Institution:||Elsevier|
|Subjects:||Oenococcus oeni; Atpase; Wine lactic acid bacteria|
|Subject (DDC):||570: Biology|
|Abstract:||The nucleotide sequences of the genes encoding the F1F0-ATPase beta-subunit from Oenococcus oeni, Leuconostoc mesenteroides subsp. mesenteroides, Pediococcus damnosus, Pediococcus parvulus, Lactobacillus brevis and Lactobacillus hilgardii were determined. Their deduced amino acid sequences showed homology values of 79-98%. Data from the alignment and ATPase tree indicated that O. oeni and L. mesenteroides subsp. mesenteroides formed a group well-separated from P. damnosus and P. parvulus and from the group comprises L. brevis and L. hilgardii. The N-terminus of the F1F0-ATPase beta-subunit of O. oeni contains a stretch of additional 38 amino acid residues. The catalytic site of the ATPase beta-subunit of the investigated strains is characterized by the two conserved motifs GGAGVGKT and GERTRE. The amplified atpD coding sequences were inserted into the pCRT7/CT-TOPO vector using TA-cloning strategy and transformed in Escherichia coli. SDS-PAGE and Western blot analyses confirmed that O. oeni has an ATPase beta-subunit protein which is larger in size than the corresponding molecules from the investigated strains.|
|Fulltext version:||Published version|
|License (according to publishing contract):||Licence according to publishing contract|
|Departement:||Life Sciences and Facility Management|
|Organisational Unit:||Institute of Chemistry and Biotechnology (ICBT)|
|Appears in collections:||Publikationen Life Sciences und Facility Management|
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Sievers, M., Uermösi, C., Fehlmann, M., & Krieger, S. (2003). Cloning, sequence analysis and expression of the F1F0- ATPase beta-subunit from wine lactic acid bacteria. Systematic and Applied Microbiology, 26(3), 350–356. https://doi.org/10.1078/072320203322497374
Sievers, M. et al. (2003) ‘Cloning, sequence analysis and expression of the F1F0- ATPase beta-subunit from wine lactic acid bacteria’, Systematic and Applied Microbiology, 26(3), pp. 350–356. Available at: https://doi.org/10.1078/072320203322497374.
M. Sievers, C. Uermösi, M. Fehlmann, and S. Krieger, “Cloning, sequence analysis and expression of the F1F0- ATPase beta-subunit from wine lactic acid bacteria,” Systematic and Applied Microbiology, vol. 26, no. 3, pp. 350–356, 2003, doi: 10.1078/072320203322497374.
Sievers, Martin, et al. “Cloning, Sequence Analysis and Expression of the F1F0- ATPase Beta-Subunit from Wine Lactic Acid Bacteria.” Systematic and Applied Microbiology, vol. 26, no. 3, 2003, pp. 350–56, https://doi.org/10.1078/072320203322497374.
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