Full metadata record
DC FieldValueLanguage
dc.contributor.authorSievers, Martin-
dc.contributor.authorUermösi, Christina-
dc.contributor.authorFehlmann, Marc-
dc.contributor.authorKrieger, Sibylle-
dc.date.accessioned2018-10-19T14:15:31Z-
dc.date.available2018-10-19T14:15:31Z-
dc.date.issued2003-
dc.identifier.issn0723-2020de_CH
dc.identifier.issn1618-0984de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/11993-
dc.description.abstractThe nucleotide sequences of the genes encoding the F1F0-ATPase beta-subunit from Oenococcus oeni, Leuconostoc mesenteroides subsp. mesenteroides, Pediococcus damnosus, Pediococcus parvulus, Lactobacillus brevis and Lactobacillus hilgardii were determined. Their deduced amino acid sequences showed homology values of 79-98%. Data from the alignment and ATPase tree indicated that O. oeni and L. mesenteroides subsp. mesenteroides formed a group well-separated from P. damnosus and P. parvulus and from the group comprises L. brevis and L. hilgardii. The N-terminus of the F1F0-ATPase beta-subunit of O. oeni contains a stretch of additional 38 amino acid residues. The catalytic site of the ATPase beta-subunit of the investigated strains is characterized by the two conserved motifs GGAGVGKT and GERTRE. The amplified atpD coding sequences were inserted into the pCRT7/CT-TOPO vector using TA-cloning strategy and transformed in Escherichia coli. SDS-PAGE and Western blot analyses confirmed that O. oeni has an ATPase beta-subunit protein which is larger in size than the corresponding molecules from the investigated strains.de_CH
dc.language.isoende_CH
dc.publisherElsevierde_CH
dc.relation.ispartofSystematic and Applied Microbiologyde_CH
dc.rightsLicence according to publishing contractde_CH
dc.subjectOenococcus oenide_CH
dc.subjectAtpasede_CH
dc.subjectWine lactic acid bacteriade_CH
dc.subject.ddc570: Biologiede_CH
dc.titleCloning, sequence analysis and expression of the F1F0- ATPase beta-subunit from wine lactic acid bacteriade_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
dc.identifier.doi10.1078/072320203322497374de_CH
dc.identifier.pmid14529177de_CH
zhaw.funding.euNode_CH
zhaw.issue3de_CH
zhaw.originated.zhawYesde_CH
zhaw.pages.end356de_CH
zhaw.pages.start350de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume26de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.webfeedMikrobiologiede_CH
Appears in collections:Publikationen Life Sciences und Facility Management

Files in This Item:
There are no files associated with this item.
Show simple item record
Sievers, M., Uermösi, C., Fehlmann, M., & Krieger, S. (2003). Cloning, sequence analysis and expression of the F1F0- ATPase beta-subunit from wine lactic acid bacteria. Systematic and Applied Microbiology, 26(3), 350–356. https://doi.org/10.1078/072320203322497374
Sievers, M. et al. (2003) ‘Cloning, sequence analysis and expression of the F1F0- ATPase beta-subunit from wine lactic acid bacteria’, Systematic and Applied Microbiology, 26(3), pp. 350–356. Available at: https://doi.org/10.1078/072320203322497374.
M. Sievers, C. Uermösi, M. Fehlmann, and S. Krieger, “Cloning, sequence analysis and expression of the F1F0- ATPase beta-subunit from wine lactic acid bacteria,” Systematic and Applied Microbiology, vol. 26, no. 3, pp. 350–356, 2003, doi: 10.1078/072320203322497374.
SIEVERS, Martin, Christina UERMÖSI, Marc FEHLMANN und Sibylle KRIEGER, 2003. Cloning, sequence analysis and expression of the F1F0- ATPase beta-subunit from wine lactic acid bacteria. Systematic and Applied Microbiology. 2003. Bd. 26, Nr. 3, S. 350–356. DOI 10.1078/072320203322497374
Sievers, Martin, Christina Uermösi, Marc Fehlmann, and Sibylle Krieger. 2003. “Cloning, Sequence Analysis and Expression of the F1F0- ATPase Beta-Subunit from Wine Lactic Acid Bacteria.” Systematic and Applied Microbiology 26 (3): 350–56. https://doi.org/10.1078/072320203322497374.
Sievers, Martin, et al. “Cloning, Sequence Analysis and Expression of the F1F0- ATPase Beta-Subunit from Wine Lactic Acid Bacteria.” Systematic and Applied Microbiology, vol. 26, no. 3, 2003, pp. 350–56, https://doi.org/10.1078/072320203322497374.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.