Publication type: Article in scientific journal
Type of review: Peer review (publication)
Title: Cloning, sequence analysis and expression of the F1F0- ATPase beta-subunit from wine lactic acid bacteria
Authors: Sievers, Martin
Uermösi, Christina
Fehlmann, Marc
Krieger, Sibylle
DOI: 10.1078/072320203322497374
Published in: Systematic and Applied Microbiology
Volume(Issue): 26
Issue: 3
Page(s): 350
Pages to: 356
Issue Date: 2003
Publisher / Ed. Institution: Elsevier
ISSN: 0723-2020
Language: English
Subjects: Oenococcus oeni; Atpase; Wine lactic acid bacteria
Subject (DDC): 570: Biology
Abstract: The nucleotide sequences of the genes encoding the F1F0-ATPase beta-subunit from Oenococcus oeni, Leuconostoc mesenteroides subsp. mesenteroides, Pediococcus damnosus, Pediococcus parvulus, Lactobacillus brevis and Lactobacillus hilgardii were determined. Their deduced amino acid sequences showed homology values of 79-98%. Data from the alignment and ATPase tree indicated that O. oeni and L. mesenteroides subsp. mesenteroides formed a group well-separated from P. damnosus and P. parvulus and from the group comprises L. brevis and L. hilgardii. The N-terminus of the F1F0-ATPase beta-subunit of O. oeni contains a stretch of additional 38 amino acid residues. The catalytic site of the ATPase beta-subunit of the investigated strains is characterized by the two conserved motifs GGAGVGKT and GERTRE. The amplified atpD coding sequences were inserted into the pCRT7/CT-TOPO vector using TA-cloning strategy and transformed in Escherichia coli. SDS-PAGE and Western blot analyses confirmed that O. oeni has an ATPase beta-subunit protein which is larger in size than the corresponding molecules from the investigated strains.
Fulltext version: Published version
License (according to publishing contract): Licence according to publishing contract
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:Publikationen Life Sciences und Facility Management

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